Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.12 extracted from

  • Hamada, M.; Otsuka, K.I.; Tanaka, N.; Ogasahara, K.; Koike, K.; Hiraoka, T.; Koike, M.
    Purification properties and subunit composition of pig heart lipoate acetyltransferase (1975), J. Biochem., 78, 187-197.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing does not cause loss of activity Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
SDS-PAGE and sedimentation equilibrium data Sus scrofa
1800000 1970000 calculation from sedimentation and diffusion constants, meniscus depletion method Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrolipoamide + acetyl-CoA Sus scrofa
-
S-acetyldihydrolipoamide + CoA
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein 23 mol of lipoic acid per mol of enzyme Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of pyruvate dehydrogenase complex, optimum catalytic stoichiometry of E1:E2:E3 is 5.2:11.5:2 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.7
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-18°C, 0.05 mM potassium phosphate buffer, pH 7.0, 0.5 mM EDTA, more than 6 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + acetyl-CoA
-
Sus scrofa S-acetyldihydrolipoamide + CoA
-
?

Subunits

Subunits Comment Organism
polymer 24 * 74000 SDS-PAGE, gel filtration in 6 M guanidine-HCl, sedimentation equilibrium in 6 M guanidine-HCl Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable up to Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.5
-
Sus scrofa