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Literature summary for 2.3.1.113 extracted from
- N-Malonyltransferases from peanut (1984), Arch. Biochem. Biophys., 235, 218-227.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | 2 mg/ml, activation | Arachis hypogaea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
malonyl-CoA | - |
Arachis hypogaea |  |
| 0.012 | - |
anthranilate | - |
Arachis hypogaea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Arachis hypogaea |
| 51000 | - |
1 * 51000, SDS-PAGE | Arachis hypogaea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arachis hypogaea | - |
peanut, cv. fastigiata ("Junagarh-11" or cultivar Shulamit) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Arachis hypogaea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Arachis hypogaea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00091 | - |
- |
Arachis hypogaea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, inactivtion after 3 months | Arachis hypogaea |
| 4°C, considerable loss of activity after several weeks | Arachis hypogaea |
| glycerol, 10%, stabilizes during storage | Arachis hypogaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonyl-CoA + anthranilate | - |
Arachis hypogaea | CoA + N-malonylanthranilate | - |
? | |
| additional information | no substrates are D-tryptophan and 3,4-dichloroaniline | Arachis hypogaea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51000, SDS-PAGE | Arachis hypogaea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arachis hypogaea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
broad | Arachis hypogaea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | most active | Arachis hypogaea |
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Release 2023.1 (January 2023)
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