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Literature summary for 2.3.1.111 extracted from

  • Rainwater, D.L.; Kolattokudy, P.E.
    Fatty acid biosynthesis in Mycobacterium tuberculosis var. bovis Bacillus Calmette-Guerin. Purification and characterization of a novel fatty acid synthase, mycocerosic acid synthase, which elongates n-fatty acyl-CoA with methylmalonyl-CoA (1985), J. Biol. Chem., 260, 616-623.
    View publication on PubMed

General Stability

General Stability Organism
stabilized by glycerol Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
bovine serum albumin
-
Mycobacterium tuberculosis
CoA 1 mM, weak inhibition Mycobacterium tuberculosis
NADPH above 0.5 mM, weak inhibition Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
238000
-
2 * 238000, SDS-PAGE Mycobacterium tuberculosis
238000
-
2 * 238000, amino acid composition Mycolicibacterium smegmatis
490000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eicosanoyl-CoA + 4 methylmalonyl-CoA + 8 NADPH + 8 H+ Mycobacterium tuberculosis fatty acid biosynthesis tetramethyl-branched mycocerosoyl-CoA + 4 CoA + 4 CO2 + 8 NADP+ + 4 H2O
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol stable in 10% glycerol Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.014
-
-
Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-20°C, glycerol-buffer, stable for at least 1 month Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eicosanoyl-CoA + 4 methylmalonyl-CoA + 4 NADPH
-
Mycobacterium tuberculosis 2,4,6,8-tetramethyloctacosanoyl-CoA + 4 CoA + 4 CO2 + 4 NADP+
-
?
eicosanoyl-CoA + 4 methylmalonyl-CoA + 8 NADPH + 8 H+ multifunctional enzyme, specific for methylmalonyl-CoA, cannot be replaced by malonyl-CoA, n-acyl-CoAs of C-6 to C-20 are substrates Mycobacterium tuberculosis tetramethyl-branched mycocerosoyl-CoA + 4 CoA + 4 CO2 + 8 NADP+ + 4 H2O
-
?
eicosanoyl-CoA + 4 methylmalonyl-CoA + 8 NADPH + 8 H+ fatty acid biosynthesis Mycobacterium tuberculosis tetramethyl-branched mycocerosoyl-CoA + 4 CoA + 4 CO2 + 8 NADP+ + 4 H2O
-
?
hexanoyl-CoA + methylmalonyl-CoA + NADPH best substrate Mycobacterium tuberculosis ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 238000, SDS-PAGE Mycobacterium tuberculosis
dimer 2 * 238000, amino acid composition Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mycobacterium tuberculosis