General Stability | Organism |
---|---|
stabilized by glycerol | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | - |
Mycobacterium tuberculosis | |
CoA | 1 mM, weak inhibition | Mycobacterium tuberculosis | |
NADPH | above 0.5 mM, weak inhibition | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
238000 | - |
2 * 238000, SDS-PAGE | Mycobacterium tuberculosis |
238000 | - |
2 * 238000, amino acid composition | Mycolicibacterium smegmatis |
490000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
eicosanoyl-CoA + 4 methylmalonyl-CoA + 8 NADPH + 8 H+ | Mycobacterium tuberculosis | fatty acid biosynthesis | tetramethyl-branched mycocerosoyl-CoA + 4 CoA + 4 CO2 + 8 NADP+ + 4 H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | stable in 10% glycerol | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.014 | - |
- |
Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-20°C, glycerol-buffer, stable for at least 1 month | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eicosanoyl-CoA + 4 methylmalonyl-CoA + 4 NADPH | - |
Mycobacterium tuberculosis | 2,4,6,8-tetramethyloctacosanoyl-CoA + 4 CoA + 4 CO2 + 4 NADP+ | - |
? | |
eicosanoyl-CoA + 4 methylmalonyl-CoA + 8 NADPH + 8 H+ | multifunctional enzyme, specific for methylmalonyl-CoA, cannot be replaced by malonyl-CoA, n-acyl-CoAs of C-6 to C-20 are substrates | Mycobacterium tuberculosis | tetramethyl-branched mycocerosoyl-CoA + 4 CoA + 4 CO2 + 8 NADP+ + 4 H2O | - |
? | |
eicosanoyl-CoA + 4 methylmalonyl-CoA + 8 NADPH + 8 H+ | fatty acid biosynthesis | Mycobacterium tuberculosis | tetramethyl-branched mycocerosoyl-CoA + 4 CoA + 4 CO2 + 8 NADP+ + 4 H2O | - |
? | |
hexanoyl-CoA + methylmalonyl-CoA + NADPH | best substrate | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 238000, SDS-PAGE | Mycobacterium tuberculosis |
dimer | 2 * 238000, amino acid composition | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |