Cloned (Comment) | Organism |
---|---|
AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS | Lavandula angustifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
tyramine | with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.018 | - |
tyramine | with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.036 | - |
tyramine | with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.057 | - |
4-coumaroyl-CoA | with tyramine, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.112 | - |
phenethylamine | with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.157 | - |
4-coumaroyl-CoA | with tryptamine, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.192 | - |
feruloyl-CoA | with tyramine, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.243 | - |
tyramine | with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.3 | - |
4-coumaroyl-CoA | with phenethylamine, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.326 | - |
caffeoyl-CoA | with tyramine, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.49 | - |
cinnamoyl-CoA | with tyramine, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Lavandula angustifolia | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50600 | - |
1 * 50600, about, LAAT1, sequence determination | Lavandula angustifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lavandula angustifolia | recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lavandula angustifolia | Q0GA14 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography | Lavandula angustifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Lavandula angustifolia | - |
Storage Stability | Organism |
---|---|
4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable | Lavandula angustifolia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + phenethylamine | - |
Lavandula angustifolia | CoA + N-(4-coumaroyl)phenethylamine | - |
? | |
4-coumaroyl-CoA + tryptamine | - |
Lavandula angustifolia | CoA + N-(4-coumaroyl)tryptamine | - |
? | |
4-coumaroyl-CoA + tyramine | - |
Lavandula angustifolia | CoA + N-(4-coumaroyl)tyramine | - |
? | |
caffeoyl-CoA + tyramine | - |
Lavandula angustifolia | CoA + N-caffeoyltyramine | - |
? | |
cinnamoyl-CoA + tyramine | - |
Lavandula angustifolia | CoA + N-cinnamoyltyramine | - |
? | |
feruloyl-CoA + tyramine | - |
Lavandula angustifolia | CoA + N-feruloyltyramine | - |
? | |
additional information | recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity | Lavandula angustifolia | ? | - |
? | |
additional information | substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid | Lavandula angustifolia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50600, about, LAAT1, sequence determination | Lavandula angustifolia |
Synonyms | Comment | Organism |
---|---|---|
AAT1 | - |
Lavandula angustifolia |
AT1 | - |
Lavandula angustifolia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Lavandula angustifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Lavandula angustifolia |