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Literature summary for 2.3.1.110 extracted from

  • Landmann, C.; Huecherig, S.; Fink, B.; Hoffmann, T.; Dittlein, D.; Coiner, H.A.; Schwab, W.
    Substrate promiscuity of a rosmarinic acid synthase from lavender (Lavandula angustifolia L.) (2011), Planta, 234, 305-320.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS Lavandula angustifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
tyramine with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.018
-
tyramine with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.036
-
tyramine with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.057
-
4-coumaroyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.112
-
phenethylamine with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.157
-
4-coumaroyl-CoA with tryptamine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.192
-
feruloyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.243
-
tyramine with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.3
-
4-coumaroyl-CoA with phenethylamine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.326
-
caffeoyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.49
-
cinnamoyl-CoA with tyramine, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Lavandula angustifolia 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50600
-
1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lavandula angustifolia recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity ?
-
?

Organism

Organism UniProt Comment Textmining
Lavandula angustifolia Q0GA14
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography Lavandula angustifolia

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Lavandula angustifolia
-

Storage Stability

Storage Stability Organism
4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable Lavandula angustifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + phenethylamine
-
Lavandula angustifolia CoA + N-(4-coumaroyl)phenethylamine
-
?
4-coumaroyl-CoA + tryptamine
-
Lavandula angustifolia CoA + N-(4-coumaroyl)tryptamine
-
?
4-coumaroyl-CoA + tyramine
-
Lavandula angustifolia CoA + N-(4-coumaroyl)tyramine
-
?
caffeoyl-CoA + tyramine
-
Lavandula angustifolia CoA + N-caffeoyltyramine
-
?
cinnamoyl-CoA + tyramine
-
Lavandula angustifolia CoA + N-cinnamoyltyramine
-
?
feruloyl-CoA + tyramine
-
Lavandula angustifolia CoA + N-feruloyltyramine
-
?
additional information recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity Lavandula angustifolia ?
-
?
additional information substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid Lavandula angustifolia ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia

Synonyms

Synonyms Comment Organism
AAT1
-
Lavandula angustifolia
AT1
-
Lavandula angustifolia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Lavandula angustifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Lavandula angustifolia