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Literature summary for 2.3.1.110 extracted from

  • Kang, S.; Kang, K.; Chung, G.C.; Choi, D.; Ishihara, A.; Lee, D.; Back, K.
    Functional analysis of the amine substrate specificity domain of pepper tyramine and serotonin N-hydroxycinnamoyltransferases (2006), Plant Physiol., 140, 704-715.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Capsicum annuum

Protein Variants

Protein Variants Comment Organism
F145Y KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 3fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin Capsicum annuum
F145Y/Y147F KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 4.2fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin Capsicum annuum
additional information a series of chimeric genes is constructed by reciprocal exchange of DNA segments between the serotonin N-hydroxycinnamoyltransferase and tyramine N-hydroxycinnamoyltransferase cDNAs. Functional characterization of the recombinant chimeric proteins revealed that the amino acid residues 129 to 165 of serotonin N-hydroxycinnamoyltransferase and the corresponding residues 125 to 160 in tyramine N-hydroxycinnamoyltransferase are critical structural determinants for amine substrate specificity Capsicum annuum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
4-coumaroyl-CoA 30°C, pH 8.5, recombinant enzyme Capsicum annuum
0.04
-
tyramine 30°C, pH 8.5, recombinant enzyme Capsicum annuum
0.053
-
feruloyl-CoA 30°C, pH 8.5, recombinant enzyme Capsicum annuum
0.17
-
tyramine 30°C, pH 8.5, mutant enzyme F145Y Capsicum annuum
0.17
-
tyramine 30°C, pH 8.5, mutant enzyme F145Y/Y147F Capsicum annuum
0.779
-
dopamine 30°C, pH 8.5, recombinant enzyme Capsicum annuum
20.98
-
serotonin 30°C, pH 8.5, mutant enzyme F145Y Capsicum annuum
24
-
tryptamine 30°C, pH 8.5, recombinant enzyme Capsicum annuum
25.44
-
serotonin 30°C, pH 8.5, mutant enzyme F145Y/Y147F Capsicum annuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28221
-
x * 28221, calculatred from nucleotide sequence Capsicum annuum

Organism

Organism UniProt Comment Textmining
Capsicum annuum Q5D8C0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information THT mRNA is expressed constitutively in all pepper organs Capsicum annuum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + tyramine
-
Capsicum annuum CoA + N-(4-coumaroyl)tyramine
-
?
caffeoyl-CoA + serotonin
-
Capsicum annuum CoA + N-caffeoylserotonin
-
?
feruloyl-CoA + dopamine
-
Capsicum annuum CoA + feruloyldopamine
-
?
feruloyl-CoA + tryptamine
-
Capsicum annuum CoA + feruloyltryptamine
-
?
feruloyl-CoA + tyramine
-
Capsicum annuum CoA + feruloyltyramine
-
?
additional information wild-type enzyme shows no activity with serotonin, mutant enzyms F145Y and F145Y/Y147F show activity with serotonin Capsicum annuum ?
-
?

Subunits

Subunits Comment Organism
? x * 28221, calculatred from nucleotide sequence Capsicum annuum

Synonyms

Synonyms Comment Organism
THT
-
Capsicum annuum
tyramine N-hydroxycinnamoyltransferase
-
Capsicum annuum