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Literature summary for 2.3.1.110 extracted from
- Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures (1997), Eur. J. Biochem., 247, 1127-1135.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | addition of 14% leads to 2fold increase of enzyme activity | Nicotiana tabacum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | 20% activity at 0.5 M | Nicotiana tabacum | |
| D-tyrosine methyl ester | - |
Nicotiana tabacum | |
| DL-tyrosine methyl ester | - |
Nicotiana tabacum | |
| L-tyrosine benzyl ester | irreversible binding to tyramine binding site | Nicotiana tabacum | |
| L-tyrosine ethyl ester | - |
Nicotiana tabacum | |
| L-tyrosine methyl ester | - |
Nicotiana tabacum | |
| L-tyrosine-tert-butyl ester | - |
Nicotiana tabacum | |
| L-tyrosineamide | - |
Nicotiana tabacum | |
| L-tyrosinol | - |
Nicotiana tabacum | |
| N-Methyltyramine | - |
Nicotiana tabacum | |
| NaCl | 20% activity at 0.5 M | Nicotiana tabacum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
feruloyl-CoA | - |
Nicotiana tabacum | |
| 0.001 | - |
sinapoyl-CoA | - |
Nicotiana tabacum | |
| 0.002 | - |
4-coumaroyl-CoA | - |
Nicotiana tabacum | |
| 0.002 | - |
isoferuloyl-CoA | - |
Nicotiana tabacum | |
| 0.0043 | - |
tyramine | - |
Nicotiana tabacum | |
| 0.0047 | - |
cinnamoyl-CoA | - |
Nicotiana tabacum | |
| 0.0113 | - |
octopamine | - |
Nicotiana tabacum | |
| 0.17 | - |
4-hydroxyphenylpropylamine | - |
Nicotiana tabacum | |
| 0.202 | - |
dopamine | - |
Nicotiana tabacum | |
| 1.57 | - |
noradrenaline | - |
Nicotiana tabacum | |
| 1.585 | - |
3-methoxytyramine | - |
Nicotiana tabacum | |
| 4.276 | - |
beta-phenylethylamine | - |
Nicotiana tabacum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
SDS-PAGE | Nicotiana tabacum |
| 48000 | - |
gel filtration | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl-CoA + tyramine | Nicotiana tabacum | - |
CoA + feruloyltyramine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
cv. Xanthi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Nicotiana tabacum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| feruloyl-CoA + tyramine = CoA + N-feruloyltyramine | mechanism | Nicotiana tabacum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
- |
Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + N-(4-coumaroyl)tyramine | - |
? | |
| caffeoyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + N-caffeoyltyramine | - |
? | |
| cinnamoyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + cinnamoyltyramine | - |
? | |
| feruloyl-CoA + (4-hydroxyphenyl)propylamine | - |
Nicotiana tabacum | CoA + feruloyl-(4-hydroxyphenyl)propylamine | - |
? | |
| feruloyl-CoA + 3-methoxytyramine | - |
Nicotiana tabacum | CoA + feruloyl-3-methoxytyramine | - |
? | |
| feruloyl-CoA + beta-phenylethylamine | - |
Nicotiana tabacum | CoA + feruloyl-beta-phenylethylamine | - |
? | |
| feruloyl-CoA + dopamine | - |
Nicotiana tabacum | CoA + feruloyldopamine | - |
? | |
| feruloyl-CoA + noradrenaline | - |
Nicotiana tabacum | CoA + feruloylnoradrenaline | - |
? | |
| feruloyl-CoA + octopamine | - |
Nicotiana tabacum | CoA + feruloyloctopamine | - |
? | |
| feruloyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + feruloyltyramine | - |
? | |
| feruloyl-CoA + tyramine | low specificity for cinnamoyl-CoA derivates and hydroxyphenethylamines | Nicotiana tabacum | CoA + feruloyltyramine | - |
? | |
| isoferuloyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + isoferuloyltyramine | - |
? | |
| sinapoyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + sinapoyltyramine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 24000, SDS-PAGE | Nicotiana tabacum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
L-tyrosine benzyl ester | - |
Nicotiana tabacum | |
| 0.018 | - |
L-tyrosine-tert-butyl ester | - |
Nicotiana tabacum | |
| 0.02 | - |
L-tyrosine ethyl ester | - |
Nicotiana tabacum | |
| 0.026 | - |
L-tyrosine methyl ester | - |
Nicotiana tabacum | |
| 0.046 | - |
L-tyrosinol | - |
Nicotiana tabacum | |
| 0.055 | - |
DL-tyrosine methyl ester | - |
Nicotiana tabacum | |
| 0.58 | - |
D-tyrosine methyl ester | - |
Nicotiana tabacum | |
| 2 | - |
L-tyrosineamide | - |
Nicotiana tabacum | |
| 2.2 | - |
N-Methyltyramine | - |
Nicotiana tabacum | |
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