Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Coomassie brilliant blue | mixed inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
acetyl-CoA | + L-N6-hydroxylysine | Escherichia coli | |
0.055 | - |
L-N6-hydroxylysine | - |
Escherichia coli | |
0.079 | - |
acetyl-CoA | + L-N5-hydroxyornithine | Escherichia coli | |
0.099 | - |
acetyl-CoA | + 1-amino-5-(hydroxyamino)pentane | Escherichia coli | |
0.101 | - |
acetyl-CoA | + N-methylhydroxylamine | Escherichia coli | |
0.317 | - |
L-N5-hydroxyornithine | - |
Escherichia coli | |
0.33 | - |
1-amino-5-(hydroxyamino)pentane | - |
Escherichia coli | |
0.396 | - |
N-Methylhydroxylamine | - |
Escherichia coli | |
1.81 | - |
acetyl-CoA | + hydroxylamine | Escherichia coli | |
329.4 | - |
hydroxylamine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Escherichia coli |
150000 | 200000 | gel filtration, native enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | enzyme is involved in the biosynthesis of aerobactin in E. coli | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
294, with plasmid pABN11 | - |
Purification (Comment) | Organism |
---|---|
with plasmid pABN11 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
N6-hydroxylysine | Escherichia coli |
Storage Stability | Organism |
---|---|
20°C, stable for several days | Escherichia coli |
4°C, stable for several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (5-hydroxyamine)pentan-1-amine | - |
Escherichia coli | CoA + N-(5-aminopentyl)-N-hydroxyacetamide | - |
? | |
acetyl-CoA + DL-N6-hydroxylysine | - |
Escherichia coli | CoA + N6-acetyl-N6-hydroxylysine | - |
? | |
acetyl-CoA + hydroxylamine | - |
Escherichia coli | CoA + N-acetylhydroxylamine | - |
? | |
acetyl-CoA + L-N5-hydroxyornithine | - |
Escherichia coli | CoA + N5-acetyl-N5-hydroxyornithine | - |
? | |
acetyl-CoA + L-N6-hydroxylysine | preferred substrate | Escherichia coli | CoA + N6-acetyl-N6-hydroxylysine | - |
? | |
acetyl-CoA + N-methylhydroxylamine | - |
Escherichia coli | CoA + N-acetyl-N-methylhydroxylamine | - |
? | |
acetyl-CoA + N5-hydroxyornithine | - |
Escherichia coli | CoA + N5-acetyl-N5-hydroxyornithine | - |
r | |
additional information | propionyl-CoA has 5% of activity compared to acetyl-CoA | Escherichia coli | ? | - |
? | |
additional information | enzyme is involved in the biosynthesis of aerobactin in E. coli | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
sharp decline above | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000385 | - |
N-Methylhydroxylamine | - |
Escherichia coli | |
0.0000477 | - |
N6-hydroxylysine | - |
Escherichia coli | |
0.0000797 | - |
1-amino-5(hydroxyamino)pentane | - |
Escherichia coli | |
0.000112 | - |
N5-hydroxyornithine | - |
Escherichia coli | |
0.00177 | - |
hydroxylamine | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00071 | - |
Coomassie brilliant blue | - |
Escherichia coli |