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Literature summary for 2.3.1.102 extracted from

  • Coy, M.; Paw, B.H.; Bindereif, A.; Neilands, J.B.
    Isolation and properties of N epsilon-hydroxylysine:acetyl coenzyme A nepsilon-transacetylase from Escherichia coli pABN11 (1986), Biochemistry, 25, 2485-2489.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Coomassie brilliant blue mixed inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
acetyl-CoA + L-N6-hydroxylysine Escherichia coli
0.055
-
L-N6-hydroxylysine
-
Escherichia coli
0.079
-
acetyl-CoA + L-N5-hydroxyornithine Escherichia coli
0.099
-
acetyl-CoA + 1-amino-5-(hydroxyamino)pentane Escherichia coli
0.101
-
acetyl-CoA + N-methylhydroxylamine Escherichia coli
0.317
-
L-N5-hydroxyornithine
-
Escherichia coli
0.33
-
1-amino-5-(hydroxyamino)pentane
-
Escherichia coli
0.396
-
N-Methylhydroxylamine
-
Escherichia coli
1.81
-
acetyl-CoA + hydroxylamine Escherichia coli
329.4
-
hydroxylamine
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, SDS-PAGE Escherichia coli
150000 200000 gel filtration, native enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli enzyme is involved in the biosynthesis of aerobactin in E. coli ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
294, with plasmid pABN11
-

Purification (Commentary)

Purification (Comment) Organism
with plasmid pABN11 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
N6-hydroxylysine Escherichia coli

Storage Stability

Storage Stability Organism
20°C, stable for several days Escherichia coli
4°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + (5-hydroxyamine)pentan-1-amine
-
Escherichia coli CoA + N-(5-aminopentyl)-N-hydroxyacetamide
-
?
acetyl-CoA + DL-N6-hydroxylysine
-
Escherichia coli CoA + N6-acetyl-N6-hydroxylysine
-
?
acetyl-CoA + hydroxylamine
-
Escherichia coli CoA + N-acetylhydroxylamine
-
?
acetyl-CoA + L-N5-hydroxyornithine
-
Escherichia coli CoA + N5-acetyl-N5-hydroxyornithine
-
?
acetyl-CoA + L-N6-hydroxylysine preferred substrate Escherichia coli CoA + N6-acetyl-N6-hydroxylysine
-
?
acetyl-CoA + N-methylhydroxylamine
-
Escherichia coli CoA + N-acetyl-N-methylhydroxylamine
-
?
acetyl-CoA + N5-hydroxyornithine
-
Escherichia coli CoA + N5-acetyl-N5-hydroxyornithine
-
r
additional information propionyl-CoA has 5% of activity compared to acetyl-CoA Escherichia coli ?
-
?
additional information enzyme is involved in the biosynthesis of aerobactin in E. coli Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
sharp decline above Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000385
-
N-Methylhydroxylamine
-
Escherichia coli
0.0000477
-
N6-hydroxylysine
-
Escherichia coli
0.0000797
-
1-amino-5(hydroxyamino)pentane
-
Escherichia coli
0.000112
-
N5-hydroxyornithine
-
Escherichia coli
0.00177
-
hydroxylamine
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00071
-
Coomassie brilliant blue
-
Escherichia coli