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Literature summary for 2.3.1.101 extracted from
- Formylmethanofuran: tetrahydromethanopterin formyltransferase from Methanosarcina barkeri. Identification of N5-formyltetrahydromethanopterin as the product (1990), FEBS Lett., 275, 226-230.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
formylmethanofuran | - |
Methanosarcina barkeri |  |
| 0.04 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri | |
| 0.05 | - |
formylmethanofuran | - |
Methanothermobacter thermautotrophicus | |
| 0.06 | - |
formylmethanofuran | - |
Methanosarcina barkeri | |
| 0.06 | - |
formylmethanofuran | - |
Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration | Methanosarcina barkeri |
| 32000 | - |
1 * 32000 SDS-PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methanosarcina barkeri | involved in the formation of methane from CO2 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methanothermobacter thermautotrophicus | involved in the formation of methane from CO2 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
- |
- |
| Methanothermobacter thermautotrophicus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| stable in presence of molecular oxygen | Methanosarcina barkeri |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina barkeri |
- |
Methanothermobacter thermautotrophicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | ternary complex type mechanism | Methanosarcina barkeri | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | ternary complex type mechanism | Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 610 | - |
- |
Methanosarcina barkeri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tricine/KOH, pH 8, stable for 1 week | Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | involved in the formation of methane from CO2 | Methanosarcina barkeri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | involved in the formation of methane from CO2 | Methanothermobacter thermautotrophicus | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000 SDS-PAGE | Methanosarcina barkeri |
| monomer | 1 * 32000 SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Methanosarcina barkeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable for more than 4 h | Methanosarcina barkeri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1970 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri | |
| 2770 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Methanosarcina barkeri |
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