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Literature summary for 2.3.1.101 extracted from

  • Breitung, J.; Börner, G.; Scholz, S.; Linder, D.; Stetter, K.O.; Thauer, R.K.
    Salt dependence, kinetic properties and catalytic mechanism of N-formylmethanofuran:tetrahydromethanopterin formyltransferase from the extreme thermophile Methanopyrus kandleri (1992), Eur. J. Biochem., 210, 971-981.
    View publication on PubMed

General Stability

General Stability Organism
presence of salts, 1.5 M, required for optimal stabilization, order of efficiency in protecting the enzyme from heat inactivation at 90°C: K2HPO4, (NH4)2SO4, KCl, NH4Cl, NaCl, Na2SO4, Na2HPO4 Methanopyrus kandleri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
formylmethanofuran
-
Methanopyrus kandleri
0.1
-
5,6,7,8-tetrahydromethanopterin
-
Methanopyrus kandleri
20
-
N-Furfurylformamide
-
Methanopyrus kandleri

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4
-
Methanopyrus kandleri
K2HPO4 no activity in absence, assay mixture contains 2 M Methanopyrus kandleri
salts absolutely dependent on the presence of phosphate or sulfate salts for activity, efficiency of activation in decreasing order: K2HPO4, (NH4)2SO4, K2SO4, Na2SO4, Na2HPO4, no activation: NaCl, KCl, NH4Cl Methanopyrus kandleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 35000, SDS-PAGE Methanopyrus kandleri

Organic Solvent Stability

Organic Solvent Comment Organism
additional information enzyme completely soluble in 80% ammonium sulfate Methanopyrus kandleri

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanopyrus kandleri

Reaction

Reaction Comment Organism Reaction ID
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin ternary complex type mechanism Methanopyrus kandleri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Methanopyrus kandleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin specific for tetrahydromethanopterin Methanopyrus kandleri methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
?
N-furfurylformamide + 5,6,7,8-tetrahydromethanopterin pseudo-substrate Methanopyrus kandleri 2-(aminomethyl)furane + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Methanopyrus kandleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
stable up to, in presence of salts Methanopyrus kandleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Methanopyrus kandleri