Cloned (Comment) | Organism |
---|---|
expressed in an Escherichia coli JM109 DELTAargA mutant | Corynebacterium glutamicum |
gene cg3035, genomic organisation, gene cg3035 has an operon structure together with gene cg3034, expression of gene cg3035 complements an argA-deficient Escherichia coli mutant JM109 strain, recombinant overexpression in Corynebacterium glutamicum strain ATTC 13032 | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | after 48 h at 30°C the mutant DELTAcg3035 exhibits poor growth on unsupplemented MM1 plates in comparison to the Corynebacterium glutamicum wild-type, indicating a bradytrophy of the mutant due to arginine limitation | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37200 | - |
x * 37200, calculated from amino acid sequence | Corynebacterium glutamicum |
37200 | - |
x * 37200, SDS-PAGE | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | Corynebacterium glutamicum | - |
CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | Corynebacterium glutamicum | ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | Corynebacterium glutamicum ATCC 13032 | ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | Corynebacterium glutamicum ATCC 13032 | - |
CoA + N-acetyl-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | CAF20762 | gene cg3035 and gene argA | - |
Corynebacterium glutamicum | Q8NM40 | - |
- |
Corynebacterium glutamicum ATCC 13032 | CAF20762 | gene cg3035 and gene argA | - |
Corynebacterium glutamicum ATCC 13032 | Q8NM40 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | - |
Corynebacterium glutamicum | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo | Corynebacterium glutamicum | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo. L-Glutamate most probably is the preferred amino acid substrate for Cg3035 | Corynebacterium glutamicum | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo | Corynebacterium glutamicum ATCC 13032 | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo. L-Glutamate most probably is the preferred amino acid substrate for Cg3035 | Corynebacterium glutamicum ATCC 13032 | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | - |
Corynebacterium glutamicum ATCC 13032 | CoA + N-acetyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37200, calculated from amino acid sequence | Corynebacterium glutamicum |
? | x * 37200, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
ArgA | - |
Corynebacterium glutamicum |
argJ | - |
Corynebacterium glutamicum |
Cg3035 | - |
Corynebacterium glutamicum |
N-acetylglutamate synthase | - |
Corynebacterium glutamicum |
NAGS | - |
Corynebacterium glutamicum |