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Literature summary for 2.3.1.1 extracted from

  • Morizono, H.; Caldovic, L.; Shi, D.; Tuchman, M.
    Mammalian N-acetylglutamate synthase (2004), Mol. Genet. Metab., 81 Suppl 1, S4-11.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain argA- Homo sapiens
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain argA-, overexpression of the preenzyme in insect cells via baculovirus infection system, 2 processed forms with different N-terminal truncations are produced Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the enzyme contains an N-terminal putative mitochondrial targeting sequence Mus musculus 5739
-
mitochondrion the enzyme contains an N-terminal putative mitochondrial targeting sequence Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-glutamate Mus musculus
-
 CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate Homo sapiens
-
 CoA + N-acetyl-L-glutamate
-
 ?
additional information Mus musculus enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity ?
-
 ?
additional information Homo sapiens enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification 2 processed forms with different N-terminal truncations are produced, i.e. a long and a short mature form Mus musculus
proteolytic modification 2 processed forms with different N-terminal truncations are produced, i.e. a long and a short mature form Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mus musculus
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate
-
 Mus musculus CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate
-
 Homo sapiens CoA + N-acetyl-L-glutamate
-
 ?
additional information enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity Mus musculus ?
-
 ?
additional information enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme possesses a carbamate kinase fold and an acyl-CoA N-acyltransferase fold Mus musculus

Synonyms

Synonyms Comment Organism
N-acetylglutamate synthase
-
 Mus musculus
N-acetylglutamate synthase
-
 Homo sapiens
NAGS
-
 Mus musculus
NAGS
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Mus musculus
8.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Mus musculus
acetyl-CoA
-
 Homo sapiens