Cloned (Comment) | Organism |
---|---|
expressed in Mycobacterium smegmatis mc2 4,517 cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M MOPS/HEPES, pH 7.5, 20% (v/v) glycerol, 8% (w/v) PEG 4000, and a 0.02 M mixture of sodium formate, ammonium acetate, sodium citrate tribasic dihydrate, sodium oxamate, and potassium sodium tartrate tetrahydrate | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
R277A | the mutant has 18% of the wild type enzyme activity | Mycobacterium tuberculosis |
R303A | the mutant has 56% of the wild type enzyme activity | Mycobacterium tuberculosis |
R97A | the mutant has 50% of the wild type enzyme activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxy-2-naphthoic acid | the potent inhibitor binds to domain II of the enzyme and inhibits its activity | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate + 2-oxoglutarate | Mycobacterium tuberculosis | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
isochorismate + 2-oxoglutarate | Mycobacterium tuberculosis H37Rv | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex S200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate + 2-oxoglutarate | - |
Mycobacterium tuberculosis | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
isochorismate + 2-oxoglutarate | - |
Mycobacterium tuberculosis H37Rv | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase | - |
Mycobacterium tuberculosis |
MenD | - |
Mycobacterium tuberculosis |
Rv0555 | - |
Mycobacterium tuberculosis |
SEPHCHC synthase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000053 | - |
wild type enzyme, at pH 8.0 and 25°C | Mycobacterium tuberculosis | dihydroxy-2-naphthoic acid | |
0.00032 | - |
mutant enzyme R303A, at pH 8.0 and 25°C | Mycobacterium tuberculosis | dihydroxy-2-naphthoic acid | |
0.00103 | - |
mutant enzyme R97A, at pH 8.0 and 25°C | Mycobacterium tuberculosis | dihydroxy-2-naphthoic acid |