Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 | Escherichia coli K-12 |
Crystallization (Comment) | Organism |
---|---|
tetragonal crystal form | Escherichia coli K-12 |
General Stability | Organism |
---|---|
2-oxoglutarate improves protein stability | Escherichia coli K-12 |
FAD improves protein stability | Escherichia coli K-12 |
thiamine diphosphate improves protein stability | Escherichia coli K-12 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Escherichia coli K-12 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P17109 | - |
- |
Purification (Comment) | Organism |
---|---|
first step His-Trap chromatography, second step ion-exchange chromatography with Q column, third step chromatography with Superdex column | Escherichia coli K-12 |
Subunits | Comment | Organism |
---|---|---|
? | dimer or tetramer | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase | - |
Escherichia coli K-12 |
MenD | - |
Escherichia coli K-12 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
melting temperature 53°C, melting temperature 57.3°C of protein complex with thiamin diphosphate, melting temperature 54°C of protein complex with 2-oxoglutarate, melting temperature 54.5°C of protein complex with FAD | Escherichia coli K-12 |