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Literature summary for 2.2.1.7 extracted from
- Analysis of 1-deoxy-D-xylulose 5-phosphate synthase activity in Grey poplar leaves using isotope ratio mass spectrometry (2010), Phytochemistry, 71, 918-922.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | in mature poplar leaves, isoprene emission is the main metabolic sink of plastidic isoprenoid intermediates. Consequently, there is lower enzymic activity in non-isoprene-emitting lines of poplar than in emitting plants as indicator of a lower demand of metabolic flux within the MEP pathway | Populus tremula x Populus alba |
| analysis | development of a sensitive assay suitable for plant extracts that is based on the decarboxylation of labeled pyruvate and detection of 13CO2 by isotope ratio mass spectrometry | Populus tremula x Populus alba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-fluoro-2-oxopropanoate | competitive, 0.1 mM, up to 57% inhibition | Populus tremula x Populus alba |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.111 | - |
pyruvate | pH 8.5, 40°C | Populus tremula x Populus alba | |
| 0.158 | - |
D-glyceraldehyde 3-phosphate | pH 8.5, 40°C | Populus tremula x Populus alba | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tremula x Populus alba | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | activity strongly depended on leaf development with higher activity in young leaves and correlated fairly well with leaf isoprene emission potential | Populus tremula x Populus alba | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + D-glyceraldehyde 3-phosphate | - |
Populus tremula x Populus alba | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Populus tremula x Populus alba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Populus tremula x Populus alba |
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Release 2023.1 (January 2023)
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