Cloned (Comment) | Organism |
---|---|
two genes dxsA and dxsB, dxsA is located in the photosynthesis cluster, both isoenzymes expressed in Escherichia coli | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
D-glyceraldehyde 3-phosphate | recombinant isoenzyme B | Rhodobacter capsulatus | |
0.15 | - |
D-glyceraldehyde 3-phosphate | recombinant isoenzyme A | Rhodobacter capsulatus | |
0.61 | - |
pyruvate | recombinant isoenzyme A, cosubstrate D-glyceraldehyde 3-phosphate | Rhodobacter capsulatus | |
3 | - |
pyruvate | recombinant isoenzyme B, cosubstrate D-glyceraldehyde 3-phosphate | Rhodobacter capsulatus | |
4.3 | - |
pyruvate | recombinant isoenzyme A, cosubstrate D-glyceraldehyde | Rhodobacter capsulatus | |
10 | - |
D-glyceraldehyde | recombinant isoenzyme B | Rhodobacter capsulatus | |
14 | - |
D-glyceraldehyde | recombinant isoenzyme A | Rhodobacter capsulatus | |
14 | - |
pyruvate | recombinant isoenzyme B, cosubstrate D-glyceraldehyde | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | recombinant isoenzyme A | Rhodobacter capsulatus | |
Mn2+ | recombinant isoenzyme A | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
histidine-tagged recombinant isoenzyme A, SDS-PAGE | Rhodobacter capsulatus |
70500 | - |
2 * 70500, histidine-tagged recombinant isoenzyme A, homodimer, gel filtration | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
histidine-tagged recombinant proteins DXP-synthase A and DXP-synthase B, expressed in Escherichia coli | Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
histidin-tagged DXP-synthase A, expressed in Escherichia coli | Rhodobacter capsulatus |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl pH 7.5, 5 mM beta-mercaptoethanol, 0.15 mM phenylmethysulfonyl fluoride, 30% glycerol, 4-6 weeks | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde | - |
Rhodobacter capsulatus | 1-deoxy-D-xylulose + CO2 | - |
? | |
pyruvate + D-glyceraldehyde 3-phosphate | - |
Rhodobacter capsulatus | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70500, histidine-tagged recombinant isoenzyme A, homodimer, gel filtration | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
DXPase | - |
Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | recombinant isoenzyme A | Rhodobacter capsulatus |