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Literature summary for 2.2.1.7 extracted from

  • Hahn, F.M.; Eubanks, L.M.; Testa, C.A.; Blagg, B.S.J.; Baker, J.A.; Poulter, C.D.
    1-Deoxy-D-xylulose 5-phosphate synthase, the gene product of open reading frame (ORF) 2816 and ORF 2895 in Rhodobacter capsulatus (2001), J. Bacteriol., 183, 1-11.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
two genes dxsA and dxsB, dxsA is located in the photosynthesis cluster, both isoenzymes expressed in Escherichia coli Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
D-glyceraldehyde 3-phosphate recombinant isoenzyme B Rhodobacter capsulatus
0.15
-
D-glyceraldehyde 3-phosphate recombinant isoenzyme A Rhodobacter capsulatus
0.61
-
pyruvate recombinant isoenzyme A, cosubstrate D-glyceraldehyde 3-phosphate Rhodobacter capsulatus
3
-
pyruvate recombinant isoenzyme B, cosubstrate D-glyceraldehyde 3-phosphate Rhodobacter capsulatus
4.3
-
pyruvate recombinant isoenzyme A, cosubstrate D-glyceraldehyde Rhodobacter capsulatus
10
-
D-glyceraldehyde recombinant isoenzyme B Rhodobacter capsulatus
14
-
D-glyceraldehyde recombinant isoenzyme A Rhodobacter capsulatus
14
-
pyruvate recombinant isoenzyme B, cosubstrate D-glyceraldehyde Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ recombinant isoenzyme A Rhodobacter capsulatus
Mn2+ recombinant isoenzyme A Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
histidine-tagged recombinant isoenzyme A, SDS-PAGE Rhodobacter capsulatus
70500
-
2 * 70500, histidine-tagged recombinant isoenzyme A, homodimer, gel filtration Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
histidine-tagged recombinant proteins DXP-synthase A and DXP-synthase B, expressed in Escherichia coli Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
histidin-tagged DXP-synthase A, expressed in Escherichia coli Rhodobacter capsulatus

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl pH 7.5, 5 mM beta-mercaptoethanol, 0.15 mM phenylmethysulfonyl fluoride, 30% glycerol, 4-6 weeks Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + D-glyceraldehyde
-
Rhodobacter capsulatus 1-deoxy-D-xylulose + CO2
-
?
pyruvate + D-glyceraldehyde 3-phosphate
-
Rhodobacter capsulatus 1-deoxy-D-xylulose 5-phosphate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70500, histidine-tagged recombinant isoenzyme A, homodimer, gel filtration Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
DXPase
-
Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 recombinant isoenzyme A Rhodobacter capsulatus