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Literature summary for 2.2.1.6 extracted from

  • Porat, I.; Vinogradov, M.; Vyazmensky, M.; Lu, C.D.; Chipman, D.M.; Abdelal, A.T.; Barak, Z.
    Cloning and characterization of acetohydroxyacid synthase from Bacillus stearothermophilus (2004), J. Bacteriol., 186, 570-574.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
sulfometuron methyl
-
Geobacillus stearothermophilus
valine
-
Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.8
-
pyruvate holoenzyme, pH 8.0, 60°C Geobacillus stearothermophilus
12
-
pyruvate large subunit, pH 8.0, 60°C Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ K0.5 value for holoenzyme 0.02 mM, for the isolated large subunit 1.3 mM Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18700
-
x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. Geobacillus stearothermophilus
63300
-
x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q8RL88 and Q8RL87 Q8RL88 i.e. large subunit IlvB, Q8RL87 i.e. small subunit IlvN
-

Renatured (Commentary)

Renatured (Comment) Organism
the isolated large subunit has about one-third the activity of the holoenzyme, but it can be reconstituted to nearly complete activity by the addition of purified small subunits Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.12
-
large subunit, pH 7.9, 55°C Geobacillus stearothermophilus
9.16
-
holoenzyme, pH 7.9, 55°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
Geobacillus stearothermophilus (S)-acetolactate + CO2
-
?
2-oxobutanoate + pyruvate
-
Geobacillus stearothermophilus 2-hydroxy-2-methyl-3-oxopentanoate + CO2
-
?

Subunits

Subunits Comment Organism
multimer x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
hlaf-life 5 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
holoenzyme Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Geobacillus stearothermophilus
thiamine diphosphate K0.5 value for holoenzyme 0.0055 mM, for the isolated large subunit 0.24 mM Geobacillus stearothermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.012
-
sulfometuron methyl holoenzyme, pH 7.9, 55°C Geobacillus stearothermophilus
0.018
-
valine holoenzyme, pH 8.0, 60°C Geobacillus stearothermophilus