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Literature summary for 2.2.1.6 extracted from

  • Liu, W.; Yuan, G.; Du, L.; Guo, W.; Li, L.; Bi, Y.; Wang, J.
    A novel Pro197Glu substitution in acetolactate synthase (ALS) confers broad-spectrum resistance across ALS inhibitors (2015), Pestic. Biochem. Physiol., 117, 31-38.
    View publication on PubMed
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
gene als, DNa and amino acid sequence determination and analysis Myosoton aquaticum

Protein Variants

Protein Variants Comment Organism
P197E site-directed mutagenesis, the mutation confers broad-spectrum resistance across ALS inhibitors. A subpopulation (WRR04) is generated and is individually homozygous for the Pro197Glu substitution. The WRR04 population exhibits broad-spectrum resistance to tribenuron (318fold), pyrithiobac sodium (over 197fold), pyroxsulam (81fold), florasulam (over 36fold) and imazethapyr (11fold). The ALS from WRR04 shows high resistance to all the tested ALS inhibitors Myosoton aquaticum
P197E the mutation leads to high resistance to inhibitors tribenuron, pyrithiobac sodium, pyroxsulam (TP, 81-fold), florasulam, and imazethapyr Myosoton aquaticum

Inhibitors

Inhibitors Comment Organism Structure
florasulam inhibitor of wild-type enzyme, poor inhibition of the P197E mutant enzyme Myosoton aquaticum
imazethapyr inhibitor of wild-type enzyme, poor inhibition of the P197E mutant enzyme Myosoton aquaticum
imidazolinone
-
 Myosoton aquaticum
additional information enzyme mutation P197E causes chick weed plant resistance to ALS inhibitors in population WRR04 Myosoton aquaticum
pyrimidinylthiobenzoate
-
 Myosoton aquaticum
pyrithiobac
-
 Myosoton aquaticum
pyrithiobac sodium inhibitor of wild-type enzyme, poor inhibition of the P197E mutant enzyme Myosoton aquaticum
pyroxsulam inhibitor of wild-type enzyme, poor inhibition of the P197E mutant enzyme Myosoton aquaticum
sulfonylaminocarbonyltriazolinone
-
 Myosoton aquaticum
sulfonylurea
-
 Myosoton aquaticum
triazolopyrimidine
-
 Myosoton aquaticum
tribenuron inhibitor of wild-type enzyme, poor inhibition of the P197E mutant enzyme Myosoton aquaticum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Myosoton aquaticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 pyruvate Myosoton aquaticum
-
 2-acetolactate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Myosoton aquaticum
-
 water chickweed
-
Myosoton aquaticum V5Q0Z4 water chickweed collected in summer of 2012 of the putative resistant population AH02 from a wheat field in Qiaotian, Longji, Tianchang, Anhui, China, where tribenuron and pyroxsulam fail to control water chickweed since 2011
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Myosoton aquaticum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Myosoton aquaticum 2-acetolactate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
acetohydroxyacid synthase
-
 Myosoton aquaticum
AHAS
-
 Myosoton aquaticum
ALS
-
 Myosoton aquaticum

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Myosoton aquaticum
thiamine diphosphate dependent on Myosoton aquaticum

General Information

General Information Comment Organism
metabolism key enzyme for the biosynthesis of branched-chain amino acids (valine, leucine and isoleucine) that are required for plant growth Myosoton aquaticum