Cloned (Comment) | Organism |
---|---|
expressed in Klebsiella pneumoniae KG-rs cells | Klebsiella pneumoniae |
gene als, cloning in Escherichia coli strain DH5alpha | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants with overexpression of 2-acetolactate synthase (ALS), 2-acetolactate decarboxylase, and acetoin reductase, either individually or in combination, are constructed to improve 2,3-butanediol production in Klebsiella pneumoniae. The strain (KG-rs) that overexpresses both 2-acetolactate synthase and acetoin reductase shows an improved 2,3-butanediol yield. When cultured in the media with five different carbon sources (glucose, galactose, fructose, sucrose, and lactose), the mutant exhibits higher 2,3-butanediol productivity and production than the parental strain in all the tested carbon sources except for lactose. The 2,3-butanediol production of strain KG-rs in a batch fermentation with glucose as the carbon source is 12% higher than that of the parental strain, overview | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | Klebsiella pneumoniae | - |
2-acetolactate + CO2 | - |
? | |
2 pyruvate | Klebsiella pneumoniae KG1 | - |
2-acetolactate + CO2 | - |
? | |
2 pyruvate | Klebsiella pneumoniae KG-1 | - |
2-acetolactate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae KG-1 | - |
- |
- |
Klebsiella pneumoniae KG1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Klebsiella pneumoniae | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Klebsiella pneumoniae KG1 | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Klebsiella pneumoniae KG-1 | 2-acetolactate + CO2 | - |
? | |
additional information | detection of nonenzymatic acetoin formation from acetolactate in the assay | Klebsiella pneumoniae | ? | - |
? | |
additional information | detection of nonenzymatic acetoin formation from acetolactate in the assay | Klebsiella pneumoniae KG1 | ? | - |
? | |
additional information | detection of nonenzymatic acetoin formation from acetolactate in the assay | Klebsiella pneumoniae KG-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
alpha-acetolactate synthase | - |
Klebsiella pneumoniae |
ALS | - |
Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | fermentation pathways in Klebsiella pneumoniae, overview. The biosynthesis route of 2,3-BD in Klebsiella pneumoniae proceeds via pyruvate, acetolactate, and acetoin to 2,3-BD. 2,3-BD production from pyruvate involves three enzymes, namely, 2-acetolactate synthase (ALS), 2-acetolactate decarboxylase (ALDC), and acetoin reductase (AR). These enzymes catalyze the production of acetolactate from pyruvate, acetoin from acetolactate, and 2,3-BD from acetoin | Klebsiella pneumoniae |