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Literature summary for 2.2.1.6 extracted from

  • Le, D.T.; Yoon, M.Y.; Tae Kim, Y.; Choi, J.D.
    Two consecutive aspartic acid residues conferring herbicide resistance in tobacco acetohydroxy acid synthase (2005), Biochim. Biophys. Acta, 1749, 103-112.
    View publication on PubMed
Search for more literature for 2.2.1.6

Protein Variants

Protein Variants Comment Organism
D374A substrate inhibition at high concentrations, strong resistance to Londax and Cadre, 10fold increase in activation efficiency for thiamine diphosphate Nicotiana tabacum
D374A/D375A strong resistance to Londax and to C, about 2fold increase in affinity for FAD, decrease in activation efficiency for thiamine diphosphate Nicotiana tabacum
D374E greatly reduced activity, strong resistance to Londax, 8fold increase in affinity for FAD, decrease in activation efficiency for thiamine diphosphate Nicotiana tabacum
D374E/D375E strong resistance to Londax and to C Nicotiana tabacum
D375A about 10fold increase in Km-value, strong resistance to Londax Nicotiana tabacum
D375E about 3fold reduction in Km-value, strong resistance to Londax, about 3fold increase in activation efficiency of FAD Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one i.e. Cadre Nicotiana tabacum
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide
-
 Nicotiana tabacum
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide ratio 1:1, i.e. Londax Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
 pyruvate mutant D375E, pH 7.5, 37°C Nicotiana tabacum
5.7
-
 pyruvate mutant D374A, pH 7.5, 37°C Nicotiana tabacum
11.7
-
 pyruvate wild-type, pH 7.5, 37°C Nicotiana tabacum
55.8
-
 pyruvate mutant D374A/D375A, pH 7.5, 37°C Nicotiana tabacum
109.3
-
 pyruvate mutant D375A, pH 7.5, 37°C Nicotiana tabacum
266.7
-
 pyruvate mutant D374E/D375E, pH 7.5, 37°C Nicotiana tabacum
557.6
-
 pyruvate mutant D374E, pH 7.5, 37°C Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
 mutant D374A/D375A, pH 7.5, 37°C Nicotiana tabacum
0.64
-
 mutant D374E/D375E, pH 7.5, 37°C Nicotiana tabacum
0.65
-
 mutant D375A, pH 7.5, 37°C Nicotiana tabacum
0.77
-
 mutant D374E, pH 7.5, 37°C Nicotiana tabacum
0.91
-
 mutant D374A, pH 7.5, 37°C Nicotiana tabacum
1.53
-
 mutant D375E, pH 7.5, 37°C Nicotiana tabacum
1.55
-
 wild-type, pH 7.5, 37°C Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Nicotiana tabacum 2-acetolactate + CO2
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Nicotiana tabacum
thiamine diphosphate
-
 Nicotiana tabacum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000013
-
 N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide wild-type, pH 7.5, 37°C Nicotiana tabacum
0.000108
-
 N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide mutant D374E, pH 7.5, 37°C Nicotiana tabacum
0.0008
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant D375A, pH 7.5, 37°C Nicotiana tabacum
0.00086
-
 N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide mutant D374A, pH 7.5, 37°C Nicotiana tabacum
0.0041
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one wild-type, pH 7.5, 37°C Nicotiana tabacum
0.0133
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant D374A/D375A, pH 7.5, 37°C Nicotiana tabacum
0.0155
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant D374E, pH 7.5, 37°C Nicotiana tabacum
0.0293
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide wild-type, pH 7.5, 37°C Nicotiana tabacum
0.0296
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant D374E/D375E, pH 7.5, 37°C Nicotiana tabacum
0.036
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant D374E, pH 7.5, 37°C Nicotiana tabacum
0.0389
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant D375E, pH 7.5, 37°C Nicotiana tabacum
0.0741
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant D374A, pH 7.5, 37°C Nicotiana tabacum
0.181
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant D374A, pH 7.5, 37°C Nicotiana tabacum
0.198
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant D375A, pH 7.5, 37°C Nicotiana tabacum
0.309
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant D374A/D375A, pH 7.5, 37°C Nicotiana tabacum
0.79
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant D374E/D375E, pH 7.5, 37°C Nicotiana tabacum