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Literature summary for 2.2.1.6 extracted from
- Properties and regulation of valine-sensitive acetolactate synthase from mitochondria of Euglena gracilis (1982), J. Gen. Microbiol., 128, 1211-1216.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | stimulates | Euglena gracilis |  |
| dithiothreitol | stimulates | Euglena gracilis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxobutanoate | inhibits formation of acetolactate from pyruvate | Euglena gracilis | |
| Ile | 5 mM, 32% inhibition | Euglena gracilis | |
| L-2-aminobutanoate | 5 mM, 21% inhibition | Euglena gracilis | |
| L-Thr | 5 mM, 6.6% inhibition | Euglena gracilis | |
| Leu | 5 mM, 23% inhibition | Euglena gracilis | |
| Val | 5 mM, 89% inhibition; feed-back inhibition; noncompetitive | Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
pyruvate | - |
Euglena gracilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Euglena gracilis | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | Euglena gracilis | key enzyme in synthesis of branched-chain amino acids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 24 h, 36% loss of activity | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Euglena gracilis | 2-acetolactate + CO2 | - |
? | |
| pyruvate | key enzyme in synthesis of branched-chain amino acids | Euglena gracilis | ? | - |
? | |
| pyruvate + 2-oxobutanoate | much higher affinity for 2-oxobutanoate than for pyruvate | Euglena gracilis | acetohydroxybutanoate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | activates | Euglena gracilis | |
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Release 2023.1 (January 2023)
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