Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-ribose-5-phosphate | above 15 mM | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0156 | - |
D-erythrose 4-phosphate | pH 7.5, 25°C | Methanocaldococcus jannaschii | |
0.0278 | - |
D-erythrose 4-phosphate | pH 7.5, 50°C | Methanocaldococcus jannaschii | |
0.64 | - |
D-fructose 6-phosphate | pH 7.5, 25°C | Methanocaldococcus jannaschii | |
0.65 | - |
D-fructose 6-phosphate | pH 7.5, 50°C | Methanocaldococcus jannaschii | |
2.2 | - |
D-ribose 5-phosphate | pH 7.5, 50°C, concentration of ribose-5-phosphate between 0.5 and 15 mM | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23960 | - |
10 * 24000, SDS-PAGE, 10 * 23960, calculated | Methanocaldococcus jannaschii |
24000 | - |
10 * 24000, SDS-PAGE, 10 * 23960, calculated | Methanocaldococcus jannaschii |
271000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
pH 7.5, 25°C | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + D-erythrose 4-phosphate | - |
Methanocaldococcus jannaschii | ? | - |
? | |
D-fructose 6-phosphate + D-ribose 5-phosphate | - |
Methanocaldococcus jannaschii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 24000, SDS-PAGE, 10 * 23960, calculated | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
3 weeks, no loss of activity | Methanocaldococcus jannaschii |
80 | - |
4 h, no loss of activity, 24 h, 50% residual activity | Methanocaldococcus jannaschii |
90 | - |
15 min, 20% loss of activity | Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Methanocaldococcus jannaschii |