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Literature summary for 2.2.1.1 extracted from

  • Meshalkina, L.E.; Solovjeva, O.N.; Khodak, Y.A.; Drutsa, V.L.; Kochetov, G.A.
    Isolation and properties of human transketolase (2010), Biochemistry (Moscow), 75, 873-880.
    View publication on PubMed
Search for more literature for 2.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information in vitro induction of the expression by arabinose decreases the amount of recombinant enzyme isolated and lowers its specific activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 D-xylulose 5-phosphate pH 7.6, 22°C Homo sapiens
0.063
-
 D-ribose 5-phosphate pH 7.6, 22°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information presence of magnesium ions, alone or in combination with thiamine diphosphate, does not affect the activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67900
-
 x * 68000, SDS-PAGE, x * 67900, calculated Homo sapiens
68000
-
 x * 68000, SDS-PAGE, x * 67900, calculated Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.64
-
 pH 7.6, 22°C Homo sapiens

Storage Stability

Storage Stability Organism
-18°C, 50 mM glycylglycine buffer, pH 7.6, no activity loss throughout several months Homo sapiens
4°C, 50 mM glycylglycine buffer, pH 7.6, stable for several days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 D-xylulose 5-phosphate reversible single substrate reaction, about 6% of the double-substrate reaction rate Homo sapiens glyceraldehyde 3-phosphate + glycolaldehyde
-
 ?
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE, x * 67900, calculated Homo sapiens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate Km value is 0.074 mM Homo sapiens