Literature summary for 2.2.1.1 extracted from

Harinarayanan, R.; Murphy, H.; Cashel, M.
Synthetic growth phenotypes of Escherichia coli lacking ppGpp and transketolase A (tktA) are due to ppGpp-mediated transcriptional regulation of tktB (2008), Mol. Microbiol., 69, 882-894.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	positive transcriptional regulation of the talA-tktB operon by ppGpp	Escherichia coli
additional information	positive transcriptional regulation of the talA-tktB operon by ppGpp. Activation of tktB transcription by ppGpp occurs both through the modulation of RpoS levels and independent of RpoS	Escherichia coli

Application

Application	Comment	Organism
additional information	ppGpp-dependent functional pathway that operates through transketolase B, and which is buffered in wild-type strain by the presence of an isozyme, transketolase A	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
construction of plasmid pHR30 using pQE80L vector for IPTG inducible TktB expression	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	on LB medium, tktB mutants show no growth defect. TktA tktB double mutant shows growth inhibition in LB medium comparable to that observed in tktA ppGpp null strains. DELTAtktB::kan mutation confers synthetic growth defects in the tktA mutant similar to that observed from ppGpp deficiency. PpGpp regulates transketolase B activity in the tktA relA256 mutant	Escherichia coli
additional information	tktA mutants are slightly slower growing on LB medium. TktA tktB double mutant shows growth inhibition in LB medium comparable to that observed in tktA ppGpp null strains. DELTAtktB::kan mutation confers synthetic growth defects in the tktA mutant similar to that observed from ppGpp deficiency. PpGpp regulates transketolase B activity in the tktA relA256 mutant	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	ppGpp deficiency lowers TktB activity	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P27302	strain derivatives of MG1655	-
Escherichia coli	P33570	strain derivatives of MG1655	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + D-fructose 6-phosphate	-	Escherichia coli	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
TktA	-	Escherichia coli
TktB	-	Escherichia coli
transketolase A	-	Escherichia coli
transketolase B	-	Escherichia coli

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Release 2023.1 (January 2023)