Application | Comment | Organism |
---|---|---|
drug development | Plasmodium falciparum transketolase shows low homology with the host enzyme, thus may serve as a novel and promising target for designing inhibitors with anti-malarial activity | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL21(DE3)Rosetta cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxyphenylpyruvate | potent inhibitor when hydroxypyruvate is used as a substrate, whereas noncompetitive inhibition with fructose-6-phosphate | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.25 | - |
fructose-6-phosphate | - |
Plasmodium falciparum | |
4.78 | - |
Hydroxypyruvate | - |
Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Plasmodium falciparum | 5829 | - |
nucleus | - |
Plasmodium falciparum | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
6 * 70000, SDS-PAGE | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
clone NF54 | - |
Purification (Comment) | Organism |
---|---|
on Ni2+-NTA resin and by ammonium sulfate precipitation | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | late trophozoite stage | Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructose-6-phosphate + hydroxypyruvate | - |
Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 70000, SDS-PAGE | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.305 | - |
p-hydroxyphenylpyruvate | with hydroxypyruvate as substrate | Plasmodium falciparum |