Literature summary for 2.2.1.1 extracted from

Miller, O.J.; Hibbert, E.G.; Ingram, C.U.; Lye, G.J.; Dalby, P.A.
Optimisation and evaluation of a generic microplate-based HPLC screen for transketolase activity (2007), Biotechnol. Lett., 29, 1759-1770.

Search for more literature for 2.2.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the host strain producing the greatest amount of transketolase activity is Escherichia coli strain XL10-gold, which produces 2.3fold more transketolase than the JM107 strain	Escherichia coli

Application

Application	Comment	Organism
analysis	establishment of a rapid microplate-based HPLC assay for transketolase, for rapidly determining substrate and product concentration suitable for optimisation of biocatalytic process conditions and screening directed evolution libraries, which can be used to determine transketolase activity with a throughput of up to 1200 samples per day, whereas the well-to-well variation from HPLC measurement is just 1.9% for the lowest activities measured	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
into plasmid pQR711 and expressed in Escherichia coli strains BL21-Gold(DE3), JM107, JM109 and XL10-Gold	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	Escherichia coli BL21-Gold(DE3) pQR711 has transketolase activity of ca. 6fold lower than JM107 pQR711 and JM109 pQR711	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydroxypyruvate + D-glyceraldehyde 3-phosphate	-	Escherichia coli	CO2 + ribulose 5-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
TktA	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Escherichia coli

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Release 2023.1 (January 2023)