Literature summary for 2.2.1.1 extracted from

Aucamp, J.P.; Martinez-Torres, R.J.; Hibbert, E.G.; Dalby, P.A.
A microplate-based evaluation of complex denaturation pathways: structural stability of Escherichia coli transketolase (2008), Biotechnol. Bioeng., 99, 1303-1310.

Search for more literature for 2.2.1.1

Application

Application	Comment	Organism
analysis	a rapid microplate-based approach for measuring the denaturation curves by intrinsic tryptophan fluorescence for simple monomeric and two-state unfolding proteins like transketolase	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
tktA gene as His-tagged version complete with its own promotor overexpressed from vector pQR791 in Escherichia coli XL10-Gold	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D381A	56fold less active than the wild-type enzyme. Shows significant destabilization of native and intermediate states of transketolase that can be monitored by changes in the urea denaturation transition mid-points (C1/2) measured by fluorescence	Escherichia coli
Y440A	700fold less active than the wild-type enzyme. Shows significant destabilization of native and intermediate states of transketolase that can be monitored by changes in the urea denaturation transition mid-points (C1/2) measured by fluorescence	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
on Ni-NTA resin	Escherichia coli

Storage Stability

Storage Stability	Organism
4°C, 250 mM Tris-HCl buffer, pH 7.5, 2 weeks	Escherichia coli

Synonyms

Synonyms	Comment	Organism
TktA	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Escherichia coli

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Release 2023.1 (January 2023)