Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-arabinose 5-phosphate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
D-xylulose 5-phosphate | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
alpha2, 2 * 69000, SDS-PAGE | Rattus norvegicus |
139000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | Rattus norvegicus | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immunoaffinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
0-4°C, partially purified, several months as precipitate in 80% ammonium sulfate | Rattus norvegicus |
0-4°C, purified, several weeks as precipitate in 80% ammonium sulfate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + D-ribose 5-phosphate | - |
Rattus norvegicus | ? | - |
? | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-ribose 5-phosphate | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
additional information | D-arabinose 5-phosphate | Rattus norvegicus | ? | - |
? | |
additional information | no acceptors with D-xylulose 5-phosphate as donor substrate: glucose 6-phosphate | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 69000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | about half-maximal activity at pH 6.5 and about 60% of maximal activity at pH 10.5 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | additional thiamine does not enhance activity | Rattus norvegicus |