Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS | Sus scrofa |
54000 | - |
alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS | Sus scrofa |
138000 | - |
meniscus depletion method | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.88 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, several days | Sus scrofa |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS | Sus scrofa |
tetramer | alpha2beta2, 2 * 27000-29000 + 2 * 52000-56000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Sus scrofa |
30 | - |
assay at | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
at pH 6.5-8.7, 20 min stable | Sus scrofa |
40 | - |
pH 8.0, activity hardly changes for 60 min | Sus scrofa |
50 | - |
t1/2: 10 min | Sus scrofa |
50 | - |
1 h stable in the presence of 4 mM thiamine diphosphate | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 7.6-7.8 | Sus scrofa |
7.8 | 8.2 | - |
Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
thiamine diphosphate dissociates from holoenzyme | Sus scrofa |
6.5 | 8.7 | 20°C, 20 min stable | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | 2 mol thiamine diphosphate per mol enzyme | Sus scrofa |