Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | no stimulation by light | Triticum aestivum | 9507 | - |
chloroplast | no stimulation by light | Spinacia oleracea | 9507 | - |
cytosol | - |
Triticum aestivum | 5829 | - |
cytosol | - |
Spinacia oleracea | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Triticum aestivum | |
Mg2+ | requirement | Spinacia oleracea | |
Mg2+ | chloroplastic, not cytosolic enzyme | Triticum aestivum | |
Mg2+ | chloroplastic, not cytosolic enzyme | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37600 | - |
4 * 37600, holoenzyme, SDS-PAGE | Triticum aestivum |
150000 | - |
gel filtration | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | Triticum aestivum | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | Spinacia oleracea | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach | - |
Triticum aestivum | - |
wheat, cv. Sappo | - |
Purification (Comment) | Organism |
---|---|
- |
Triticum aestivum |
- |
Spinacia oleracea |
Renatured (Comment) | Organism |
---|---|
thiamine diphosphate reconstitutes dissociated holoenzyme | Triticum aestivum |
thiamine diphosphate reconstitutes dissociated holoenzyme | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Triticum aestivum | - |
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
- |
Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-erythrose 4-phosphate | - |
Triticum aestivum | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-erythrose 4-phosphate | - |
Spinacia oleracea | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-erythrose 4-phosphate | Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | D-erythrose 4-phosphate | Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | sedoheptulose 7-phosphate | Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | sedoheptulose 7-phosphate | Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Spinacia oleracea |
dimer | apoenzyme, gel filtration after 2-mercaptoethanol incubation | Triticum aestivum |
More | catalytically active apoenzyme dimer and holoenzyme tetramer | Triticum aestivum |
More | tetramer dissociates into dimers in the absence of thiamine diphosphate or in dilute solution | Triticum aestivum |
tetramer | 4 * 37600, holoenzyme, SDS-PAGE | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Triticum aestivum |
7.5 | 8.5 | - |
Spinacia oleracea |
8.5 | 9 | - |
Triticum aestivum |
8.5 | 9 | - |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Triticum aestivum | |
thiamine diphosphate | - |
Spinacia oleracea |