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Literature summary for 2.1.3.9 extracted from

  • Shi, D.; Yu, X.; Roth, L.; Morizono, H.; Tuchman, M.; Allewell, N.M.
    Structures of N-acetylornithine transcarbamoylase from Xanthomonas campestris complexed with substrates and substrate analogs imply mechanisms for substrate binding and catalysis (2006), Proteins, 64, 532-542.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in binary complex with its substrates, carbamoyl phosphate or N-acetyl-L-ornithine, and in ternary complex with carbamoyl phosphate and N-acetyl-L-norvaline, hanging drop vapour diffusion method, preparation by mixing of 0.002 ml of 12 mg/ml protein solution with an equal volume of reservoir solution containing the ligands, cryoprotection, X-ray diffraction structure determination and analysis at 1.8-1.95 A resolution, modeling of the transition state complex Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + N2-acetyl-L-ornithine Xanthomonas campestris
-
phosphate + N-acetyl-L-citrulline
-
?
additional information Xanthomonas campestris the enzyme is essential in the arginine biosynthesis in several eubacteria ?
-
?

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
-
-
-

Reaction

Reaction Comment Organism Reaction ID
carbamoyl phosphate + N2-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline substrate binding and catalytic mechanism, modeling of the transition state complex Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + N2-acetyl-L-ornithine
-
Xanthomonas campestris phosphate + N-acetyl-L-citrulline
-
?
additional information the enzyme is essential in the arginine biosynthesis in several eubacteria Xanthomonas campestris ?
-
?

Synonyms

Synonyms Comment Organism
AOTCase
-
Xanthomonas campestris
More the enzyme is a member of the transcarbamoylase superfamily Xanthomonas campestris
N-acetylornithine transcarbamoylase
-
Xanthomonas campestris