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Literature summary for 2.1.3.7 extracted from

  • Brewer, S.J.; Taylor, P.M.; Turner, M.K.
    An adenosine triphosphate-dependent carbamoylphosphate--3-hydroxymethylcephem O-carbamoyltransferase from Streptomyces clavuligerus (1980), Biochem. J., 185, 555-564.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.7

General Stability

General Stability Organism
phosphate anions stabilize during purification Streptomyces clavuligerus

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
 Streptomyces clavuligerus
adenosine-alpha,beta-methylene triphosphate
-
 Streptomyces clavuligerus
adenosine-beta,gamma-methylene triphosphate
-
 Streptomyces clavuligerus
ATP above 1 mM Streptomyces clavuligerus
Ba2+ weak Streptomyces clavuligerus
Cu2+
-
 Streptomyces clavuligerus
diphosphate
-
 Streptomyces clavuligerus
Fe2+ weak Streptomyces clavuligerus
NaCl
-
 Streptomyces clavuligerus
Pb2+ weak Streptomyces clavuligerus
Sn2+
-
 Streptomyces clavuligerus
Zn2+
-
 Streptomyces clavuligerus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Streptomyces clavuligerus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation, maximal activity in the presence of Mn2+ and ATP Streptomyces clavuligerus
Mn2+ activation, maximal activity in the presence of Mg2+ and ATP Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate Streptomyces clavuligerus catalyzes the synthesis of a wide range of carbamoyloxymethylcephems, e.g. cefuroxime phosphate + a 3-carbamoyloxymethylcephem
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces clavuligerus

Storage Stability

Storage Stability Organism
-10°C, 50 mM phosphate buffer, pH 7.0, stable for several weeks Streptomyces clavuligerus
4°C, in the presence of ATP and carbamoyl phosphate, stable for at least 3 days Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + 7-aminodeacetylcephalosporanic acid
-
 Streptomyces clavuligerus 7-beta-acylamino-3-carbamoyloxymethylcephem + phosphate
-
 ?
carbamoyl phosphate + 7beta-acylamino-3-hydroxymethylcephem
-
 Streptomyces clavuligerus 7beta-acylamino-3-carbamoyloxymethylcephem + phosphate
-
 ?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate catalyzes the synthesis of a wide range of carbamoyloxymethylcephems, e.g. cefuroxime Streptomyces clavuligerus phosphate + a 3-carbamoyloxymethylcephem
-
 ?
carbamoyl phosphate + decarbamoylcefuroxime
-
 Streptomyces clavuligerus carbamoylcefuroxime + phosphate
-
 ?
carbamoyl phosphate + [3-hydroxymethyl]deacetylcephalosporin C ATP may be a substrate or an effector Streptomyces clavuligerus 7beta-(5-D-aminoadipamido)-3-carbamoyloxymethylceph-3-em-4-carboxylic acid + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Streptomyces clavuligerus

pH Range

pH Minimum pH Maximum Comment Organism
5.9 7.4 about 75% of maximal activity at pH 5.9 and 7.4 Streptomyces clavuligerus

Cofactor

Cofactor Comment Organism Structure
ATP activation, up to 1 mM, may be substrate or effector, maximal activity in the presence of Mg2+ and Mn2+ Streptomyces clavuligerus
dATP activation, can replace ATP to some extent Streptomyces clavuligerus