Cloned (Comment) | Organism |
---|---|
enzyme protomer structure, overview | Enterococcus faecalis |
Crystallization (Comment) | Organism |
---|---|
purified full-length enzyme or in complex with inhibitors N-(phosphonoacetyl)-putrescine or N-(phosphonoacetyl)-L-ornithine, and trunacted enzyme in complex with N-(phosphonoacetyl)-L-ornithine, hanging drop vapor diffusion technique, mixing of 0.001 ml of 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5 containing 0.43 mM ligand with 0.001 ml of crystallization solution composed of 125 mM (NH4)2SO4, 17% PEG 3350, 0.1 M Bis-Tris, pH 5.5, 21°C, X-ray diffraction structure determination and analysis at 2.5 A, 2.0 A, and 1.59 A resolution, respectively, modeling | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | confirmation of decreased stability of the trimer of the enzyme lacking the C-terminal helix by deleting this helix | Enterococcus faecalis |
R54G | site-directed mutagenesis, inactive mutant, not exhibiting any PTC or OTC activity | Enterococcus faecalis |
Y230V/G231S/L232M/Y233G | engineering of the 230-loop of the enzyme, by replacing the sequence 230YGLY233 of the putrescine signature by its OTC counterpart VSMG, favors the use of ornithine and impairs that of putrescine | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
delta-N-(phosphonoacetyl)-L-ornithine | bisubstrate analogue inhibitor | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
L-ornithine | recombinant enzyme mutant Y230V/G231S/L232M/Y233G, pH 8.5, 37°C | Enterococcus faecalis | |
36.4 | - |
L-ornithine | recombinant wild-type enzyme, pH 8.5, 37°C | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120300 | - |
trimeric enzyme, gel filtration | Enterococcus faecalis |
230000 | - |
hexameric enzyme, gel filtration | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | Enterococcus faecalis | - |
L-citrulline + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q837U7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24 | - |
recombinant wild-type enzyme, substrate L-ornithine, pH 8.5, 37°C | Enterococcus faecalis |
82 | - |
recombinant enzyme mutant Y230V/G231S/L232M/Y233G, substrate L-ornithine, pH 8.5, 37°C | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Enterococcus faecalis | L-citrulline + phosphate | - |
r | |
additional information | in addition to using putrescine, see EC 2.1.3.6, the enzyme can utilize L-ornithine as a poor substrate. Differences between the respective 230 and SMG loops of putrescine transcarbamoylase PTC and OTC appear to account for the differential preference of these enzymes for putrescine and ornithine, active center and the discrimination mechanism between putrescine and ornithine, overview | Enterococcus faecalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | two trimer structure | Enterococcus faecalis |
More | presence or absence of supratrimeric oligomerization, structure comparison and analysis, overview | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
ornithine transcarbamylase | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Enterococcus faecalis |
General Information | Comment | Organism |
---|---|---|
additional information | sequence 230YGLY233 is the putrescine signature sequence | Enterococcus faecalis |
physiological function | the OTC activity of the enzyme is involved in arginine biosynthesis | Enterococcus faecalis |