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Literature summary for 2.1.3.3 extracted from

  • Shabalin, I.G.; Porebski, P.J.; Cooper, D.R.; Grabowski, M.; Onopriyenko, O.; Grimshaw, S.; Savchenko, A.; Chruszcz, M.; Minor, W.
    Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 A resolution (2012), Acta Crystallogr. Sect. F, 68, 1018-1024.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.3

Cloned(Commentary)

Cloned (Comment) Organism
gene argF, expression of His-tagged aOTC in Escherichia coli strain BL21-CodonPlus(DE3)-RIL Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded aOTC, hanging drop vapor diffusion method, mixing of 500 nl 10 mg/ml protein solution with 500 nl well solution containing 25% PEG 3350, 0.2 M NaCl, 0.1 M HEPES, pH 8.0, room temperature, 1 week, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement and modeling Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + L-ornithine Campylobacter jejuni
-
 L-citrulline + phosphate
-
 ?
carbamoyl phosphate + L-ornithine Campylobacter jejuni NCTC 11168
-
 L-citrulline + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q9PNU6 subsp. jejuni, gene argF
-
Campylobacter jejuni NCTC 11168 Q9PNU6 subsp. jejuni, gene argF
-

Purification (Commentary)

Purification (Comment) Organism
gene argF, expression of His-tagged aOTC in Escherichia coli strain BL21-CodonPlus(DE3)-RIL Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
 Campylobacter jejuni L-citrulline + phosphate
-
 ?
carbamoyl phosphate + L-ornithine the mechanism of the aOTC-catalyzed reaction involves nucleophilic attack of the electron pair of the epsilon-amino group of L-ornithine on the carbonyl group of carbamoyl phosphate Campylobacter jejuni L-citrulline + phosphate
-
 ?
carbamoyl phosphate + L-ornithine
-
 Campylobacter jejuni NCTC 11168 L-citrulline + phosphate
-
 ?
carbamoyl phosphate + L-ornithine the mechanism of the aOTC-catalyzed reaction involves nucleophilic attack of the electron pair of the epsilon-amino group of L-ornithine on the carbonyl group of carbamoyl phosphate Campylobacter jejuni NCTC 11168 L-citrulline + phosphate
-
 ?

Subunits

Subunits Comment Organism
trimer forms a head-to-head pseudohexamer in the asymmetric unit, each monomer is composed of an N-terminal CP-binding domain and a C-terminal ORN-binding domain joined by two interdomain helices. Conformation of the B2-H3 loop, residues 68-78, is involved in binding CP in an adjacent subunit of the trimer, overview Campylobacter jejuni

Synonyms

Synonyms Comment Organism
anabolic ornithine transcarbamoylase
-
 Campylobacter jejuni
AOTC
-
 Campylobacter jejuni
ornithine carbamoyltransferase
-
 Campylobacter jejuni
OTC
-
 Campylobacter jejuni

General Information

General Information Comment Organism
evolution aOTC is a widespread enzyme that is found in a large variety of organisms from bacteria to mammals Campylobacter jejuni
metabolism anabolic ornithine transcarbamoylase is involved in the urea cycle and L-arginine biosynthesis Campylobacter jejuni
additional information the conserved active site of Campylobacter jejuni aOTC consists of residues from both carbamoyl phosphate binding and L-ornithine-binding domains of the subunit and the B2-H3 loop, residues 68-78, from an adjacent subunit of the trimer, active site structure, overview Campylobacter jejuni
physiological function OTC catalyzes the reversible transfer of the carbamoyl group from carbamoyl phosphate to the Nepsilon atom of L-ornithine to produce L-citrulline. There are two types of enzyme: anabolic, aOTC, and catabolic, cOTC. Anabolic OTCs catalyze the forward reaction and participate in the urea cycle and L-arginine biosynthesis Campylobacter jejuni