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Literature summary for 2.1.3.3 extracted from

  • Xu, Y.; Feller, G.; Gerday, C.; Glansdorff, N.
    Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in L-ornithine carbamoyltransferase from Moritella abyssi (2003), J. Bacteriol., 185, 2161-2168.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.3

Inhibitors

Inhibitors Comment Organism Structure
Delta-N-Phosphonoacetyl-L-ornithine weak Moritella abyssi
L-ornithine
-
 Moritella abyssi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 Carbamoyl phosphate pH 9.0, 30°C Moritella abyssi
1
-
 Carbamoyl phosphate pH 9.0, 20°C Moritella abyssi
1.1
-
 Carbamoyl phosphate pH 9.0, 5°C Moritella abyssi
1.78
-
 L-ornithine pH 9.0, 5°C Moritella abyssi
3.34
-
 L-ornithine pH 9.0, 10°C Moritella abyssi
5.67
-
 L-ornithine pH 9.0, 15°C Moritella abyssi
8
-
 L-ornithine pH 9.0, 20°C Moritella abyssi
22.49
-
 L-ornithine pH 9.0, 25°C Moritella abyssi
45
-
 L-ornithine pH 9.0, 30°C Moritella abyssi

Organism

Organism UniProt Comment Textmining
Moritella abyssi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
 Moritella abyssi phosphate + L-citrulline
-
 ?

Subunits

Subunits Comment Organism
dodecamer
-
 Moritella abyssi
More in presence of up to 1 M Nacl, amount of dodecamer increases Moritella abyssi
trimer
-
 Moritella abyssi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 25
-
 Moritella abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5
-
 37% residual activity Moritella abyssi
50
-
 potassium phosphate pH 7.0, half-life 60 min, PIPES-NaOH pH 7.0, half-life 14 min, Tris-HCl, pH 7.5, half-life 9 min Moritella abyssi
55
-
 potassium phosphate pH 7.0, half-life 40 min, PIPES-NaOH pH 7.0, half-life 4.5 min, Tris-HCl, pH 7.5, half-life 3 min Moritella abyssi
60
-
 potassium phosphate pH 7.0, half-life 2 min Moritella abyssi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
235
-
 L-ornithine pH 9.0, 5°C Moritella abyssi
382
-
 L-ornithine pH 9.0, 10°C Moritella abyssi
546
-
 L-ornithine pH 9.0, 15°C Moritella abyssi
690
-
 L-ornithine pH 9.0, 30°C Moritella abyssi
694
-
 L-ornithine pH 9.0, 20°C Moritella abyssi
750
-
 L-ornithine pH 9.0, 25°C Moritella abyssi

pH Range

pH Minimum pH Maximum Comment Organism
9 10
-
 Moritella abyssi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0115
-
 Delta-N-Phosphonoacetyl-L-ornithine pH 9.0, 20°C Moritella abyssi