Cloned (Comment) | Organism |
---|---|
expression of wild-type, Y227C, A240D, E277G, Y227C/E277G and A240D/E277G mutant enzyme in Saccharomyces cerevisiae | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
A240D | 10fold increase of Km at 55°C | Pyrococcus furiosus |
E277G | 14fold increase of Km at 55°C | Pyrococcus furiosus |
Y227D | slight increase of Km at 55°C | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, Mono Q, arginine-Sepharose column | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Pyrococcus furiosus | phosphate + L-citrulline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
370 | - |
ornithine | at 30°C | Pyrococcus furiosus | |
500 | - |
ornithine | at 55°C | Pyrococcus furiosus | |
2200 | - |
ornithine | Y227C/E277G double mutant, at 30°C | Pyrococcus furiosus | |
2900 | - |
ornithine | A240D/E277G double mutant, at 30°C | Pyrococcus furiosus | |
3500 | - |
ornithine | Y227C/E277G double mutant, at 55°C | Pyrococcus furiosus | |
4300 | - |
ornithine | A240D/E277G double mutant, at 55°C | Pyrococcus furiosus |