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Literature summary for 2.1.3.3 extracted from

  • Morizono, H.; Tuchman, M.; Rajagopal, B.S.; McCann, M.T.; Listrom, C.D.; Yuan, X.; Venugopal, D.; Barany, G.; Allewell, N.M.
    Expression, purification and kinetic characterization of wild-type human ornithine transcarbamylase and a recurrent mutant that produces 'late onset' hyperammonemia (1997), Biochem. J., 322, 625-631.
No PubMed abstract available
Search for more literature for 2.1.3.3

Activating Compound

Activating Compound Comment Organism Structure
additional information activity increases with ionic strength up to 100 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
coexpression with GroES and GroEL in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
R277W shows no substrate inhibition by ornithine, 70fold lower affinity for L-ornithine Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Homo sapiens
dithiothreitol
-
 Homo sapiens
ornithine recombinant wild-type enzyme Homo sapiens
Zn2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 ornithine transcarbamoyltransferase deficiency is the most common cause of inherited urea cycle disorders
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and R277W mutant enzyme, ammonium sulfate, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.2
-
 recombinant enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris acetate, 2 mM EDTA, pH 7.4, 50% glycerol, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
 Homo sapiens phosphate + L-citrulline
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51
-
 R277W mutant enzyme, 50% loss of activity Homo sapiens
56
-
 recombinant enzyme, 50% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 gradual loss of activity above pH 8.5 or below pH 6.5 Homo sapiens