Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamine | - |
Rattus norvegicus | |
histidine | - |
Rattus norvegicus | |
L-2-aminobutyrate | - |
Rattus norvegicus | |
L-isoleucine | - |
Rattus norvegicus | |
L-leucine | - |
Rattus norvegicus | |
L-lysine | weak | Rattus norvegicus | |
L-norvaline | - |
Rattus norvegicus | |
L-valine | - |
Rattus norvegicus | |
methionine | - |
Rattus norvegicus | |
additional information | not inhibited by arginine | Rattus norvegicus | |
phosphate | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39600 | - |
3 * 39600, SDS-PAGE, sedimentation equilibrium analysis in 6 M guanidine-HCl | Rattus norvegicus |
39900 | - |
3 * 39900, amino acid sequence | Rattus norvegicus |
112000 | - |
sedimentation equilibrium analysis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
885 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, 2% loss of activity per month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Rattus norvegicus | phosphate + L-citrulline | - |
? | |
carbamoyl phosphate + lysine | 4.3% of the rate with ornithine | Rattus norvegicus | phosphate + homocitrulline | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 39600, SDS-PAGE, sedimentation equilibrium analysis in 6 M guanidine-HCl | Rattus norvegicus |
trimer | 3 * 39900, amino acid sequence | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
triethanolamine/acetate buffer | Rattus norvegicus |
7.9 | - |
HEPES/NaOH buffer | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.5 | approx. 50% of maximal activity at pH 6.7 and 8.5, triethanolamine/acetate buffer | Rattus norvegicus |
7.2 | 8.3 | 71% of maximal activity at pH 7.2, 94% of maximal activity at pH 8.3, HEPES/NaOH buffer | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | 9 | 60 min, stable | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
L-norvaline | - |
Rattus norvegicus | |
0.25 | - |
phosphate | - |
Rattus norvegicus | |
0.58 | - |
L-alpha-aminobutyrate | - |
Rattus norvegicus | |
1.2 | - |
L-leucine | - |
Rattus norvegicus | |
10.5 | - |
L-isoleucine | - |
Rattus norvegicus | |
12.5 | - |
L-valine | - |
Rattus norvegicus | |
40 | - |
L-histidin | - |
Rattus norvegicus |