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Literature summary for 2.1.3.3 extracted from

  • Szilagyi, I.; Vargha, G.; Szabo, G.
    Ornithine carbamoyltransferase of Streptomyces fradiae: purification and properties (1987), FEMS Microbiol. Lett., 48(1), 115-119.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
L-2-aminobutyrate 5-20 mM; L-isomer Streptomyces fradiae
ornithine above 8 mM Streptomyces fradiae
Zn2+ 5-20 mM Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, enzyme may be a dimer, SDS-PAGE Streptomyces fradiae
87000
-
gel filtration Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, aminohexyl-agarose Streptomyces fradiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
Streptomyces fradiae
500
-
-
Streptomyces fradiae

Storage Stability

Storage Stability Organism
0-4°C, 2 months, no loss of activity Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
Streptomyces fradiae phosphate + L-citrulline
-
?

Subunits

Subunits Comment Organism
? x * 40000, enzyme may be a dimer, SDS-PAGE Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10
-
Streptomyces fradiae

pH Range

pH Minimum pH Maximum Comment Organism
7 10 sharp decrease in activity below pH 9.0 Streptomyces fradiae