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Literature summary for 2.1.3.3 extracted from
- Ornithine transcarbamylase from Streptococcus faecalis and bovine liver. I. Isolation and subunit structure (1972), J. Biol. Chem., 247, 1641-1653.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37800 | - |
3 * 37800, SDS-PAGE | Bos taurus |
| 39600 | - |
6 * 39600, SDS-PAGE | Enterococcus faecalis |
| 108000 | - |
sedimentation equilibrium measurement | Bos taurus |
| 223000 | - |
sedimentation equilibrium measurement | Enterococcus faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Enterococcus faecalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate | Bos taurus |
| ammonium sulfate, streptomycin, DEAE-cellulose, heating, DEAE-Sephadex | Enterococcus faecalis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| effect of pH and temperature on the rate of renaturation of guanidine-HCl dissociated enzyme, approx. 90% recovery at 25°C and pH 7.0 | Bos taurus |
| effect of pH and temperature on the rate of renaturation of guanidine-HCl dissociated enzyme, approx. 90% recovery at 25°C and pH 7.0 | Enterococcus faecalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 780 | - |
- |
Bos taurus |
| 3200 | - |
- |
Enterococcus faecalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| enzyme suspension in 3.0 M ammonium sulfate, pH 5.0, 3 years, no loss of activity | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-ornithine | - |
Bos taurus | phosphate + L-citrulline | - |
? |  |
| carbamoyl phosphate + L-ornithine | - |
Enterococcus faecalis | phosphate + L-citrulline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 39600, SDS-PAGE | Enterococcus faecalis |
| trimer | 3 * 37800, SDS-PAGE | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
stable at low ionic strength, gradual loss of activity in 1 M KCl | Enterococcus faecalis |
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Release 2023.1 (January 2023)
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