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Literature summary for 2.1.3.2 extracted from

  • Vickrey, J.F.; Herve, G.; Evans, D.R.
    Pseudomonas aeruginosa aspartate transcarbamoylase. Characterization of its catalytic and regulatory properties (2002), J. Biol. Chem., 277, 24490-24498.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-(Phosphonoacetyl)-L-aspartate at concentrations below 0.000002 mM, inhibition above Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method, equal volumes of 1 mg/ml enzyme in 100 mM Tris-HCl, pH 8.5 is mixed with 100 mM citrate, pH 5, and 10% 6K polyethylene glycol Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
ATP excess MgCl2 abolishes inhibition Pseudomonas aeruginosa
CTP
-
Pseudomonas aeruginosa
GTP
-
Pseudomonas aeruginosa
UTP
-
Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
Carbamoyl phosphate
-
Pseudomonas aeruginosa
2.6
-
L-aspartate
-
Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
6 * 45000 + 6 * 36000, SDS-PAGE Pseudomonas aeruginosa
45000
-
6 * 45000 + 6 * 36000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Pseudomonas aeruginosa
-
phosphate + N-carbamoyl-L-aspartate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, Ni2+-affinity column Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
450
-
recombinant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
?
carbamoylphosphate + L-aspartate
-
Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
?

Subunits

Subunits Comment Organism
dodecamer 6 * 45000 + 6 * 36000, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 steep increase in activity until pH 8.5, decrase above Pseudomonas aeruginosa