Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.3.2 extracted from

  • Jacobson, G.R.; Stark, G.R.
    Aspartate transcarbamylases (1973), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 9, 225-308.
No PubMed abstract available
Search for more literature for 2.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
adenosine very weak activation Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray structure at 5.5 A resolution Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-ThioUMP
-
 Escherichia coli
CTP 800-1000 mM urea lower or eliminate CTP inhibition Escherichia coli
CTP
-
 Halobacterium salinarum
CTP
-
 Pseudomonas aeruginosa
CTP
-
 Pseudomonas fluorescens
CTP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
cytidine
-
 Escherichia coli
diphosphate
-
 Enterococcus faecalis
diphosphate
-
 Escherichia coli
diphosphate
-
 Pseudomonas fluorescens
GTP
-
 Escherichia coli
GTP
-
 Pseudomonas aeruginosa
GTP
-
 Vigna radiata var. radiata
Inosine
-
 Escherichia coli
ITP weak inhibition Escherichia coli
N-(Phosphonoacetyl)-L-aspartate
-
 Bacillus subtilis
N-(Phosphonoacetyl)-L-aspartate
-
 Escherichia coli
N-(Phosphonoacetyl)-L-aspartate
-
 Mus musculus
N-(Phosphonoacetyl)-L-aspartate
-
 Vigna radiata var. radiata
N-methyl phosphonoacetamide
-
 Escherichia coli
nucleotides
-
 Citrobacter freundii
nucleotides
-
 Escherichia coli
nucleotides
-
 Pseudomonas aeruginosa
nucleotides
-
 Pseudomonas fluorescens
phosphonoacetamide
-
 Escherichia coli
UTP weak inhibition Escherichia coli
UTP
-
 Pseudomonas aeruginosa
UTP
-
 Pseudomonas fluorescens
UTP
-
 Saccharomyces cerevisiae
UTP
-
 Vigna radiata var. radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ regulatory subunit contains 6 Zn2+ per 300000 Da protein, Zn2+ may be replaced by Hg2+ or Cd2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
-
 Vigna radiata var. radiata
93000
-
 250000 Da and 93000 Da isoforms Citrobacter freundii
100000
-
 100000 Da and 300000 Da isofomrms Pseudomonas vulgaris
120000 140000
-
 Enterococcus faecalis
250000
-
 250000 Da and 93000 Da isoforms Citrobacter freundii
300000
-
 100000 Da and 300000 Da isoforms Salmonella enterica subsp. enterica serovar Typhimurium
300000
-
 100000 Da and 300000 Da isoforms Pseudomonas aeruginosa
300000
-
 100000 Da and 300000 Da isoforms Serratia marcescens
300000
-
 100000 Da and 300000 Da isoforms Azotobacter vinelandii
300000
-
 100000 Da and 300000 Da isoforms Pseudomonas vulgaris
310000
-
 sedimentation equilibrium Escherichia coli
360000
-
-
 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Salmonella enterica subsp. enterica serovar Typhimurium
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Vigna radiata var. radiata
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Bacillus subtilis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Mus musculus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Rattus norvegicus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Saccharomyces cerevisiae
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Triticum aestivum
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Oryctolagus cuniculus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Neurospora crassa
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Pseudomonas fluorescens
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Klebsiella aerogenes
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Pseudomonas aeruginosa
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Halobacterium salinarum
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Serratia marcescens
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Enterococcus faecalis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Azotobacter vinelandii
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Lactuca sativa
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Citrobacter freundii
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Pseudomonas vulgaris
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Escherichia coli catalyzes the formation of carbamoyl-L-aspartate, the first compound unique to the biosynthetic pathway for pyrimidine nucleotides phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Bacillus subtilis
-
-
-
Citrobacter freundii
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Halobacterium salinarum
-
-
-
Klebsiella aerogenes
-
-
-
Lactuca sativa
-
-
-
Mus musculus
-
-
-
Neurospora crassa
-
-
-
Oryctolagus cuniculus
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas vulgaris
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Serratia marcescens
-
-
-
Triticum aestivum
-
 wheat
-
Vigna radiata var. radiata
-
 mung bean
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Vigna radiata var. radiata
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Bacillus subtilis
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Mus musculus
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Escherichia coli
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Rattus norvegicus
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Saccharomyces cerevisiae
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Triticum aestivum
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Oryctolagus cuniculus
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Neurospora crassa
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Pseudomonas fluorescens
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Klebsiella aerogenes
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Pseudomonas aeruginosa
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Halobacterium salinarum
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Serratia marcescens
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Enterococcus faecalis
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Azotobacter vinelandii
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Lactuca sativa
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Citrobacter freundii
a
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Pseudomonas vulgaris
a

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Oryctolagus cuniculus
-
germ
-
 Vigna radiata var. radiata
-
germ
-
 Triticum aestivum
-
seedling
-
 Vigna radiata var. radiata
-
seedling
-
 Lactuca sativa
-
spleen
-
 Mus musculus
-
spleen
-
 Rattus norvegicus
-
spleen
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Bacillus subtilis phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Mus musculus phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Rattus norvegicus phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Saccharomyces cerevisiae phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Triticum aestivum phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Oryctolagus cuniculus phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Neurospora crassa phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Pseudomonas fluorescens phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Klebsiella aerogenes phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Halobacterium salinarum phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Serratia marcescens phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Azotobacter vinelandii phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Lactuca sativa phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Citrobacter freundii phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Pseudomonas vulgaris phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Bacillus subtilis phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Mus musculus phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Rattus norvegicus phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Saccharomyces cerevisiae phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Triticum aestivum phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Oryctolagus cuniculus phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Neurospora crassa phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Pseudomonas fluorescens phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Klebsiella aerogenes phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Halobacterium salinarum phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Serratia marcescens phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Azotobacter vinelandii phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Lactuca sativa phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Citrobacter freundii phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Pseudomonas vulgaris phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate catalyzes the formation of carbamoyl-L-aspartate, the first compound unique to the biosynthetic pathway for pyrimidine nucleotides Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
More
-
 Serratia marcescens
More 2 distinct kinds of separable subunits, a catalytically active C subunit of 33000 Da and a regulatory R subunit of 17000 Da, native structure: (C3)2(R2)3 i.e. a dimer of C subunits and a trimer of R subunits Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bacillus subtilis
8.5
-
-
 Pseudomonas fluorescens
8.5
-
 maxima at pH 8.5 and pH 10.2 Escherichia coli
10.2
-
-
 Vigna radiata var. radiata
10.2
-
 maxima at pH 8.5 and pH 10.2 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP activates Escherichia coli
dATP stimulates Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000027
-
 N-(phosphonacetyl)-L-aspartate
-
 Escherichia coli
0.037
-
 CTP
-
 Escherichia coli
0.09
-
 diphosphate
-
 Escherichia coli
0.66
-
 phosphonoacetamide
-
 Escherichia coli
1.54
-
 N-methyl phosphonoacetamide
-
 Escherichia coli