Any feedback?
Literature summary for 2.1.3.1 extracted from
- The 5S subunit of transcarboxylase interacts with free biotin as studied by transferred-NOESY and Saturation Transfer Difference NMR (2008), Protein Pept. Lett., 15, 624-629.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the 5S subunit protein is expressed as intein fusion protein in pTXB1 vector at the N-terminus in BL21DE3 Escherichia coli cells | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
1.3S subunit, biotinyl subunit, 12 per complex | Propionibacterium freudenreichii subsp. shermanii |
| 55000 | - |
native recombinant protein after intein tag cleavage | Propionibacterium freudenreichii subsp. shermanii |
| 95000 | - |
5S subunit, recombinant fusion protein, 505 amino acids | Propionibacterium freudenreichii subsp. shermanii |
| 110000 | - |
5S subunit, outer subunit, six per complex | Propionibacterium freudenreichii subsp. shermanii |
| 330000 | - |
12S subunit, central hexameric core | Propionibacterium freudenreichii subsp. shermanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-methylmalonyl-CoA + pyruvate | Propionibacterium freudenreichii subsp. shermanii | - |
propanoyl-CoA + oxaloacetate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on chitin column beads, the intein tag is removed by auto-splicing | Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-methylmalonyl-CoA + pyruvate | - |
Propionibacterium freudenreichii subsp. shermanii | propanoyl-CoA + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | - |
Propionibacterium freudenreichii subsp. shermanii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5S subunit of transcarboxylase | - |
Propionibacterium freudenreichii subsp. shermanii |
| transcarboxylase | - |
Propionibacterium freudenreichii subsp. shermanii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | - |
Propionibacterium freudenreichii subsp. shermanii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
