Cloned (Comment) | Organism |
---|---|
1.3S subunit cloned and expressed in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
5S subunit cloned and expressed in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
Crystallization (Comment) | Organism |
---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | loss of assembly-promoting factor activity after digestion with trypsin, V8 protease, pronase | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
2.3 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
43 | - |
recombinant 5S subunit + assembly-promotin factor, overall reaction | Propionibacterium freudenreichii subsp. shermanii |
48 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionyl-CoA + oxaloacetate | two partial reactions | Propionibacterium freudenreichii subsp. shermanii | (S)-methylmalonyl-CoA + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | existence of a novel, previously unidentified subunit, a assemply-promoting factor, APF, which is nessessary for the assemply of enzyme, MW: 60000 Da, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
More | the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 | Propionibacterium freudenreichii subsp. shermanii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assembly-promoting factor heat stable | Propionibacterium freudenreichii subsp. shermanii |