Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.2.9 extracted from

  • Lewandowski, T.; Huang, J.; Fan, F.; Rogers, S.; Gentry, D.; Holland, R.; Demarsh, P.; Aubart, K.; Zalacain, M.
    Staphylococcus aureus formyl-methionyl transferase mutants demonstrate reduced virulence factor production and pathogenicity (2013), Antimicrob. Agents Chemother., 57, 2929-2936.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.2.9

Inhibitors

Inhibitors Comment Organism Structure
SB-734453
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet Staphylococcus aureus
-
 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet Staphylococcus aureus WCUH29
-
 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
 hospital-acquired methicillin-resistant strain
-
Staphylococcus aureus WCUH29
-
 hospital-acquired methicillin-resistant strain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Staphylococcus aureus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Staphylococcus aureus WCUH29 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Synonyms

Synonyms Comment Organism
FMT
-
 Staphylococcus aureus
formyl-methionyl transferase
-
 Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate
-
 Staphylococcus aureus

General Information

General Information Comment Organism
malfunction formyl-methionyl transferase mutants demonstrate reduced virulence factor production and pathogenicity. Loss-of-function mutations in the enzyme impart pleiotropic effects that include a reduced growth rate, a nonhemolytic phenotype, and a drastic reduction in production of multiple extracellular proteins, including key virulence factors, such as alpha-hemolysin and Panton-Valentine leukocidin, that have been associated with Staphylococcus aureus pathogenicity. Consequently, Staphylococcus aureus formyl-methionyl transferase mutants are greatly attenuated in neutropenic and nonneutropenic murine pyelonephritis infection models and show very high survival rates compared with wild-type Staphylococcus aureus. FMT-null mutants have a more severe fitness cost than previously anticipated, leading to a substantial loss of pathogenicity and a restricted ability to produce an invasive infection. Lack of transformylase affects the production of additional virulence factors, with reductions in total extracellular proteins of at least 50% observed in supernatants of cultures from the different Staphylococcus aureus WCUH29 FMT mutants Staphylococcus aureus
physiological function protein synthesis initiates with formyl-methionyl-tRNAi, and therefore, all newly synthesized polypeptides contain an N-formyl-methionine terminal end that, in most cases, is not retained in mature proteins Staphylococcus aureus