Cloned (Comment) | Organism |
---|---|
gene encoding mitochondrial enzyme is cloned and expressed in Escherichia coli BL21(DE3) as His-tagged enzyme | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
bovine mitochondrial L-methionyl-tRNA | - |
Bos taurus | |
0.000097 | - |
E. coli L-methionyl-tRNAmMet | - |
Bos taurus | |
0.0001 | - |
E. coli L-methionyl-tRNAfMet | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | translational initiation in mitochondria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3) | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + bovine mitochondrial L-methionyl-tRNA | - |
Bos taurus | tetrahydrofolate + bovine mitochondrial N-formylmethionyl-tRNA | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet | - |
Bos taurus | tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet | - |
Bos taurus | tetrahydrofolate + E. coli N-formylmethionyl-tRNAmMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | broader substrate specificity than that of E. coli enzyme | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
additional information | translational initiation in mitochondria | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Bos taurus |