Cloned (Comment) | Organism |
---|---|
gene 42 encoding enzyme is sequenced and expressed in Escherichia coli | Tequatrovirus T4 |
Crystallization (Comment) | Organism |
---|---|
crystal structure in the presence of substrate dCMP at 1.6 A resolution | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
D179N | mutant with altered substrate preference, favoring dUMP rather than dCMP | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28450 | - |
2 * 28450, homodimer of 246-residue subunits | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | enzyme forms part of phage DNA protection system | ? | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | involved in biosynthesis of DNA precursor | ? | |
additional information | Tequatrovirus T4 | enzyme functions in vivo as a component of a multienzyme complex called dNTP synthetase | ? | - |
? | |
additional information | Tequatrovirus T4 | enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | P08773 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of His6-tagged enzyme | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | catalytic mechanism | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | enzyme forms part of phage DNA protection system | ? | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | involved in biosynthesis of DNA precursor | ? | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | enzyme structure, dCMP binding site, folate cofactor binding site, way of substrate binding in the active site, key catalytic residue Cys-148 acts as nucleophile to attack the C6-atom of the cytosine base | Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? | |
additional information | enzyme functions in vivo as a component of a multienzyme complex called dNTP synthetase | Tequatrovirus T4 | ? | - |
? | |
additional information | enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus | Tequatrovirus T4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28450, homodimer of 246-residue subunits | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | as cofactor | Tequatrovirus T4 | |
5,10-methylenetetrahydrofolate | weak binding of the folate cofactor to enzyme | Tequatrovirus T4 | |
tetrahydrofolate | - |
Tequatrovirus T4 |