Cloned (Comment) | Organism |
---|---|
structural gene 42 encodes enzyme | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
D179A | mutant not inactivated by 5-fluoro-dUMP | Tequatrovirus T4 |
D179N | mutant with altered substrate preference, favoring dUMP rather than dCMP | Tequatrovirus T4 |
D179N | mutation of active site, 10fold increased rate of inhibition by 5-fluoro-dUMP | Tequatrovirus T4 |
D179S | mutant not inactivated by 5-fluoro-dUMP | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorodeoxyuridylate | inactivates by formation of a covalent complex containing enzyme, inhibitor and methylenetetrahydrofolate in a time-dependent manner, 1 mol 5-fluoro-dUMP per enzyme monomer, no substantial inhibition of wild-type enzyme in vivo, inhibition mechanism | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28600 | - |
x * 28600 | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
Escherichia coli JM101 infected with bacteriophage T4 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | mechanism | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Cys-148 is catalytically essential | Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 28600 | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Tequatrovirus T4 | |
tetrahydrofolate | - |
Tequatrovirus T4 |