Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | low specific activity samples can be activated by a 30 min room temperature incubation in 50 mM DTT | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | complete loss of activity, second-order kinetics, inactivation is reversed by dithiothreitol, inactivation involves the modification of one thiol group per mol of dimeric enzyme | Tequatrovirus T4 | |
aminopterin | competitive inhibitor with respect to tetrahydrofolate | Tequatrovirus T4 | |
dihydrofolate | competitive inhibitor with respect to tetrahydrofolate | Tequatrovirus T4 | |
methotrexate | competitive inhibitor with respect to tetrahydrofolate | Tequatrovirus T4 | |
N-ethylmaleimide | complete loss of activity, second-order kinetics, pH-dependent inactivation, dCMP protects against inactivation, dCMP plus either methotrexate or aminopterin greatly enhances protection, inactivation involves the modification of one thiol group per mol of dimeric enzyme | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
tetrahydrofolate | - |
Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, two equally sized subunits are arranged asymmetrically, SDS-PAGE | Tequatrovirus T4 |
51400 | - |
gel filtration | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
Escherichia coli strains Cr 63 and B infected with bacteriophage T4 | - |
Purification (Comment) | Organism |
---|---|
1026fold purification | Tequatrovirus T4 |
Escherichia coli B infected with | Tequatrovirus T4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Tequatrovirus T4 |
Storage Stability | Organism |
---|---|
frozen, 20 mM KH2PO4, pH 6.8, 6 months | Tequatrovirus T4 |
room temperature, 24 h, stable | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | stoichiometry of dCMP binding to the dimeric enzyme is 1:1 and depends upon a reduced thiol group, one thiol-containing active site per mol of dimeric enzyme, sequential order of substrate binding | Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, two equally sized subunits are arranged asymmetrically, SDS-PAGE | Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Tequatrovirus T4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
room temperature, 24 h, stable | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | active cofactor is likely 5,10-methylenetetrahydrofolate | Tequatrovirus T4 | |
tetrahydrofolate | - |
Tequatrovirus T4 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
aminopterin | - |
Tequatrovirus T4 | |
0.016 | - |
methotrexate | - |
Tequatrovirus T4 | |
0.021 | - |
dihydrofolate | - |
Tequatrovirus T4 |