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Literature summary for 2.1.2.5 extracted from

  • Jeanguenin, L.; Lara-Nunez, A.; Pribat, A.; Mageroy, M.H.; Gregory, J.F.; Rice, K.C.; de Crecy-Lagard, V.; Hanson, A.D.
    Moonlighting glutamate formiminotransferases can functionally replace 5-formyltetrahydrofolate cycloligase (2010), J. Biol. Chem., 285, 41557-41566.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-CHO-THF cycloligase deletion mutant with strain MG1655 background Streptococcus pyogenes
glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background Thermoplasma acidophilum
glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background Picrophilus torridus
glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-N-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background Candidatus Koribacter versatilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Picrophilus torridus
0.0007
-
N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Picrophilus torridus
0.0018
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Candidatus Koribacter versatilis
0.0037
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Thermoplasma acidophilum
0.0038
-
N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Thermoplasma acidophilum
0.0048
-
N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Streptococcus pyogenes
0.0054
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Streptococcus pyogenes
0.0058
-
N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Candidatus Koribacter versatilis
0.034
-
L-glutamate pH 7.3, 30°C Picrophilus torridus
0.113
-
L-glutamate pH 7.3, 30°C Thermoplasma acidophilum
0.206
-
L-glutamate pH 7.3, 30°C Candidatus Koribacter versatilis
1.03
-
L-glutamate pH 7.3, 30°C Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-formimidoyltetrahydrofolate + L-glutamate Thermoplasma acidophilum
-
tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate Streptococcus pyogenes
-
tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate Picrophilus torridus
-
tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate Candidatus Koribacter versatilis
-
tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate Candidatus Koribacter versatilis Ellin345
-
tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate Streptococcus pyogenes M1
-
tetrahydrofolate + N-formimidoyl-L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Candidatus Koribacter versatilis
-
-
-
Candidatus Koribacter versatilis Ellin345
-
-
-
no activity in Herpetosiphon aurantiacus
-
the gene encoding glycine formimidoyltransferase is an inactive pseudogene, the histidine utilization hut operon in Herpetosiphon aurantiacus is entirely broken up, suggesting that this pathway no longer functions
-
Picrophilus torridus
-
-
-
Streptococcus pyogenes
-
-
-
Streptococcus pyogenes M1
-
-
-
Thermoplasma acidophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Thermoplasma acidophilum
recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Streptococcus pyogenes
recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Picrophilus torridus
recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Candidatus Koribacter versatilis

Storage Stability

Storage Stability Organism
-80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, loss of 80% activity after 8 weeks Candidatus Koribacter versatilis
-80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks Thermoplasma acidophilum
-80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks Streptococcus pyogenes
-80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-formimidoyltetrahydrofolate + L-glutamate
-
Thermoplasma acidophilum tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate
-
Streptococcus pyogenes tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate
-
Picrophilus torridus tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate
-
Candidatus Koribacter versatilis tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate
-
Candidatus Koribacter versatilis Ellin345 tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
5-formimidoyltetrahydrofolate + L-glutamate
-
Streptococcus pyogenes M1 tetrahydrofolate + N-formimidoyl-L-glutamate
-
?
N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
Thermoplasma acidophilum N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
?
N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
Streptococcus pyogenes N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
?
N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
Picrophilus torridus N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
?
N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
Candidatus Koribacter versatilis N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
?
N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
Candidatus Koribacter versatilis Ellin345 N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
?
N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
Streptococcus pyogenes M1 N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
?

Synonyms

Synonyms Comment Organism
glutamate formiminotransferase
-
Thermoplasma acidophilum
glutamate formiminotransferase
-
Streptococcus pyogenes
glutamate formiminotransferase
-
Picrophilus torridus
glutamate formiminotransferase
-
Candidatus Koribacter versatilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Thermoplasma acidophilum
30
-
assay at Streptococcus pyogenes
30
-
assay at Picrophilus torridus
30
-
assay at Candidatus Koribacter versatilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0022
-
L-glutamate pH 7.3, 30°C Streptococcus pyogenes
0.0022
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Streptococcus pyogenes
0.0045
-
L-glutamate pH 7.3, 30°C Thermoplasma acidophilum
0.0045
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Thermoplasma acidophilum
0.0092
-
L-glutamate pH 7.3, 30°C Candidatus Koribacter versatilis
0.0092
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Candidatus Koribacter versatilis
0.0193
-
L-glutamate pH 7.3, 30°C Picrophilus torridus
0.0193
-
5-formimidoyltetrahydrofolate pH 7.3, 30°C Picrophilus torridus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Thermoplasma acidophilum
7.3
-
assay at Streptococcus pyogenes
7.3
-
assay at Picrophilus torridus
7.3
-
assay at Candidatus Koribacter versatilis

General Information

General Information Comment Organism
metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Thermoplasma acidophilum
metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Streptococcus pyogenes
metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Picrophilus torridus
metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Candidatus Koribacter versatilis
physiological function utilization of supplied 5-formyltetrahydrofolate by Streptococcus pyogenes requires expression of the native glutamate formiminotransferase Streptococcus pyogenes