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Literature summary for 2.1.2.2 extracted from
- Evaluation of the kinetic mechanism of Escherichia coli glycinamide ribonucleotide transformylase probed by site-directed mutagenesis and pH-dependent studies (1999), Biochemistry, 38, 10024-10031.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0211 | - |
N10-formyl-5,8-dideazafolate | wild-type | Escherichia coli |  |
| 0.0211 | - |
N10-formyl-5,8-dideazafolate | strain H108A | Escherichia coli | |
| 0.0284 | - |
N10-formyl-5,8-dideazafolate | strain D144A | Escherichia coli | |
| 0.095 | - |
beta-glycinamide ribonucleotide | wild-type | Escherichia coli | |
| 0.099 | - |
N10-formyl-5,8-dideazafolate | strain H121Q | Escherichia coli | |
| 0.115 | - |
beta-glycinamide ribonucleotide | strain H108A | Escherichia coli | |
| 0.122 | - |
beta-glycinamide ribonucleotide | strain D144A | Escherichia coli | |
| 0.414 | - |
beta-glycinamide ribonucleotide | strain H121Q | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Escherichia coli | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | pH-dependent dimer-monomer transition near physiological pH might act as a means of regulating enzyme activity | Escherichia coli |
| monomer | 1 * 23000 | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 8.8 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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